Bone marrow-derived mesenchymal cells can rescue osteogenic capacity of devitalized autologous bone
Bone marrow-derived mesenchymal cells can rescue osteogenic capacity of devitalized autologous bone
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DOI：
10.1002/term.67
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发表时间：
2008-01-01
影响因子：
3.3
通讯作者：
Takakura, Yoshinori
中科院分区：
文献类型：
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作者：
Tohma, Yasuaki;Ohgushi, Hajime;Takakura, Yoshinori
在临床上，许多骨科医生都面临着骨脆性的问题，如骨肿瘤灭活治疗后的骨折、病理性骨折和转移瘤等。本研究的目的是确定失活自体骨的成骨能力的丧失是否可以使用培养的骨髓间充质细胞来挽救。以大鼠股骨为材料，建立放射性灭活骨模型，分为完整骨组、放射性骨组和放射性骨与培养间充质细胞联合组。将每个骨皮下移植到同系大鼠中。在移植后2或4周，进行生化分析[碱性磷酸酶（ALP）活性和骨钙素mRNA表达]和组织学测量。此外，我们验证了新形成的骨的起源，使用性别决定区Y（sry）基因作为区分供体和受体的标记。在完整骨和含间充质细胞的照射骨中，检测到ALP活性和骨钙素mRNA表达，并且在组织学上清楚地看到活的成骨细胞和新形成的骨。此外，从头形成的骨的来源的分析表明，在照射的骨与间充质细胞的新形成的骨来自培养的骨髓来源的间充质细胞。这些结果证明，灭活自体骨的成骨能力可以使用组织工程技术挽救。该手术有助于各种临床治疗，如局部转移性肿瘤，灭活治疗后的病理性骨折和宽缘肿瘤切除后的重建。受益将适用于所有类型的失活骨。版权所有（c）2008约翰威利父子有限公司。
In clinical cases, many orthopaedists have been troubled with bone fragility, such as fractures after devitalization therapy for bone tumour, pathological fractures and metastatic tumours. The aim of this study was to determine whether loss of osteogenic capacity of devitalized autologous bones can be rescued using cultured bone marrow-derived mesenchymal cells. A devitalized bone model was produced from rat femur by irradiation and three groups were prepared: intact bone, irradiated bone and irradiated bone combined with cultured mesenchymal cells. Each bone was transplanted subcutaneously into a syngeneic rat. At 2 or 4 weeks after transplantation, biochemical analyses [alkaline phosphatase (ALP) activity and osteocalcin mRNA expression] and histological measurement were performed. Moreover, we verified the origin of newly formed bone, using the sex-determining region Y (sry) gene as a marker to distinguish between donor and recipient. In both intact bone and irradiated bone with mesenchymal cells, ALP activity and osteocalcin mRNA expression were detected and living osteoblasts together with newly formed bone were clearly seen histologically. Furthermore, analysis of the origin of de novo formed bone indicated that newly formed bone in irradiated bone with mesenchymal cells was derived from cultured bone marrow-derived mesenchymal cells. These results proved that the osteogenic capacity of devitalized autologous bone can be rescued using tissue-engineering techniques. This procedure should contribute to various clinical treatments, such as local metastatic tumours, pathological fracture after devitalization therapy and reconstruction after wide-margin tumour resection. The benefits would be applicable to all types of devitalized bone. Copyright (c) 2008 John Wiley & Sons, Ltd.