Small-diameter blood vessels engineered with bone marrow-derived cells
Small-diameter blood vessels engineered with bone marrow-derived cells
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DOI：
10.1097/01.sla.0000154268.12239.ed
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发表时间：
2005-03-01
影响因子：
9
通讯作者：
Kim, BS
中科院分区：
文献类型：
--
作者：
Cho, SW;Lim, SH;Kim, BS
目的：本研究的目的是研究骨髓源性细胞（BMCs）是否能再生血管组织并改善组织工程小直径（内径= 3mm）血管移植物的通畅性。背景资料：bmc已被证明具有分化为内皮样细胞和血管平滑肌样细胞的能力，可能为血管组织工程提供另一种细胞来源。因此，我们组织工程化小直径血管移植物与bmc和脱细胞动脉。方法：将犬bmc体外分化为平滑肌α -肌动蛋白/平滑肌球蛋白重链阳性细胞和血管性血变因子/ cd31阳性细胞，并植入内径为3mm的脱细胞犬颈动脉。将种子移植物植入细胞供体犬体内。分别用免疫组化和血管造影观察血管组织再生和移植物通畅程度。结果：在犬颈动脉介入模型中，植入bmc的血管移植物可保持通畅长达8周，而未植入bmc的血管移植物可在2周内闭塞。移植后8周内，血管内皮、中膜和外膜3种成分再生。植入前用荧光染料标记的bmc在移植血管中被检测到，表明bmc参与了血管组织的再生。结论：BMCs在组织工程小直径血管移植中具有再生血管组织和改善血管通畅的潜力。这是第一个以bmc为细胞源的小直径新血管工程的报道。
Objective: The objective of this study is to investigate if bone marrow-derived cells (BMCs) regenerate vascular tissues and improve patency in tissue-engineered small-diameter (internal diameter = 3 mm) vascular grafts.Summary Background Data: BMCs have demonstrated the ability to differentiate into endothelial-like cells and vascular smooth muscle-like cells and may offer an alternative cell source for vascular tissue engineering. Thus, we tissue-engineered small-diameter vascular grafts with BMCs and decellularized arteries.Methods: Canine BMCs were differentiated in vitro into smooth muscle alpha-actin/smooth muscle myosin heavy-chain-positive cells and von Willebrand factor/CD31-positive cells and seeded onto decellularized canine carotid arteries (internal diameter = 3 mm). The seeded grafts were implanted in cell donor dogs. The vascular-tissue regeneration and graft patency were investigated with immunohistochemistry and angiography, respectively.Results: The vascular grafts seeded with BMCs remained patent for up to 8 weeks in the canine carotid artery interposition model, whereas nonseeded grafts occluded within 2 weeks. Within 8 weeks after implantation, the vascular grafts showed regeneration of the 3 elements of artery (endothelium, media, and adventitia). BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular grafts, indicating that the BMCs participated in the vascular tissue regeneration.Conclusions: Here we show that BMCs have the potential to regenerate vascular tissues and improve patency in tissue-engineered small-diameter vascular grafts. This is the first report of a small-diameter neovessel engineered with BMCs as a cell source.