Timing of the potential of micromere‐descendants in echinoid embryos to induce endoderm differentiation of mesomere‐descendants
Timing of the potential of micromere‐descendants in echinoid embryos to induce endoderm differentiation of mesomere‐descendants
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海胆胚胎中微粒后代诱导中粒后代内胚层分化的潜力的时机
DOI：
10.1046/j.1440-169x.1999.00453.x
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发表时间：
1999
期刊：
影响因子：
4.6
通讯作者：
S. Amemiya
中科院分区：
文献类型：
--
作者：
T. Minokawa;S. Amemiya
据报道，海胆胚胎的微团有可能在 16 或 32 细胞阶段诱导动物帽中粒中的 archenteron 重组。在本研究中，进行了实验以确定微米粒传递其诱导信号的确切时期，以将中粒的细胞命运重新指定为内中胚层。将动物帽与四组微球或分离培养的微球后代重组，形成重组胚胎。微球后代在不同的发育阶段被完全去除，导致胚胎仅由中粒后代组成，并在有限的时间内受到微球的诱导影响。培养所得胚胎并检查其分化内胚层的潜力。结果表明，在中粒后代中有效诱导原肠细胞的信号来自孵化前后早期囊胚阶段的微粒后代。在此阶段之前，微米体和微米体后代稍微表现出这种潜力或根本没有表现出这种潜力。即使细胞是单独培养的，感应信号也几乎按时从微粒子孙中发出。微粒后代在重组后2小时内完成了诱导动物帽中原肠菌的信号传递。 28细胞阶段和32细胞阶段后2小时之间的动物帽可以与来自微粒后代的诱导信号发生反应。仅由动物帽中粒组成的胚胎在有限的时间内接受了来自微粒后代的感应信号，有可能发育成 8 臂 plutei。每个鞭毛体基本上在幼虫体的左侧形成成虫雏形，并变态为具有五放射对称性的幼虫。
It has been reported that the micromeres of echinoid embryos have the potential to induce an archenteron in animal cap mesomeres recombined at the 16‐ or 32‐cell stage. In the present study, experiments were performed to determine the exact period when the micromeres transmit their inductive signal to respecify the cell fate of mesomeres as endo‐mesoderm. An animal cap was recombined with a quartet of micromeres, or micromere‐descendants cultured in isolation, to form a recombinant embryo. The micromere‐descendants were completely removed at various developmental stages, resulting in an embryo composed only of mesomere‐descendants that had been under the inductive influence of micromeres for a limited period. The resulting embryos were cultured and examined for their potential to differentiate endoderm. The results indicated that the signal effective for inducing an archenteron in mesomere‐descendants emanated from the micromere‐descendants at the early blastula stage around hatching onward. Before this stage, the micromeres and micromere‐descendants showed this potential slightly or not at all. The inductive signal emanated from the micromere‐descendants almost on time even when the cells were cultured in isolation. The micromere‐descendants completed transmission of the signal for inducing the archenteron in the animal cap within 2 h of recombination. The animal cap at between the 28‐cell stage and 2 h after the 32‐cell stage could react with the inductive signal from the micromere‐descendants. Embryos composed of only animal cap mesomeres that had received the inductive signal from micromere‐descendants for a limited period had the potential to develop into 8‐armed plutei. Each pluteus formed an adult rudiment essentially on the left side of the larval body, and metamorphosed into a juvenile with pentaradiate symmetry.
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细胞相互作用和组织质量对海胆中粒分化的影响。
DOI：
10.1242/dev.109.3.625
发表时间：
1990
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Khaner,O;Wilt,F
通讯作者：
Wilt,F
海胆胚胎中的细胞相互作用和中胚层细胞命运。
DOI：
--
发表时间：
1992
期刊：
Development (Cambridge, England). Supplement
影响因子：
--
作者：
Ettensohn,CA
通讯作者：
Ettensohn,CA
不同海胆物种外胚层的自主和非自主分化。
DOI：
10.1242/dev.121.5.1497
发表时间：
1995
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Wikramanayake,AH;Brandhorst,BP;Klein,WH
通讯作者：
Klein,WH
海胆胚胎中胚层细胞的相互作用：成骨次级间充质细胞的特性。
DOI：
10.1242/dev.117.4.1275
发表时间：
1993
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Ettensohn,CA;Ruffins,SW
通讯作者：
Ruffins,SW
海胆胚胎的正常植物板规格需要微粒。
DOI：
10.1242/dev.121.10.3215
发表时间：
1995
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Ransick,A;Davidson,EH
通讯作者：
Davidson,EH