Human bladder carcinoma cell lines as indicators of oncogenic change relevant to urothelial neoplastic progression.
Human bladder carcinoma cell lines as indicators of oncogenic change relevant to urothelial neoplastic progression.
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DOI：
10.1038/bjc.1995.394
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发表时间：
1995-09
影响因子：
8.8
通讯作者：
Summerhayes, I. C.
中科院分区：
文献类型：
--
作者：
Rieger, K. M.;Little, A. F.;Swart, J. M.;Kastrinakis, W. V.;Fitzgerald, J. M.;Hess, D. T.;Libertino, J. A.;Summerhayes, I. C.
人类肿瘤细胞系的分析，以前导致新的转化相关元件的鉴定，并提供了一个有用的工具，不同基因的功能研究。为了建立这种细胞系作为尿路上皮癌相关变化指标的实用性，我们在一组人膀胱癌细胞系中表征了五种基因（p53，MDM2，Rb，E-钙粘蛋白，APC）的表达。使用单链构象多态性（SSCP）和直接测序，在7/15（47%）的细胞系中发现了p53突变，反映了膀胱肿瘤中报告的事件。免疫组织化学分析p53在培养的细胞和石蜡包埋的部分异种移植物从细胞系面板显示不一致的结果。缺乏p53核染色与EJ中的外显子5突变和J82中发现的多个p53突变相关。在蛋白质印迹分析中，在不存在可检测突变的情况下，两个p53染色阳性的细胞系显示MDM2（PSI，HT1197）的过表达。在5/15（TCCRNA、SCaBER、5637、HT 1376、J82）细胞系中记录到Rb表达缺失或异常。在5/15个细胞系（TCC17、EJ、KK 47、UM-UC-3、J82）中记录到E-钙粘蛋白的缺失，在CUBIII的免疫沉淀的E-钙粘蛋白复合物中α-连环蛋白的损失。APC的Western印迹分析揭示了1/15（CUBIII）细胞系中的截短蛋白。这组人膀胱癌细胞系中致癌事件的表征建立了膀胱肿瘤中观察到的变化的代表性，并更好地定义了这些肿瘤进展的实验性人细胞模型中的基因型背景。
Analysis of human tumour-derived cell lines has previously resulted in the identification of novel transformation-related elements and provided a useful tool for functional studies of different genes. To establish the utility of such cell lines as indicators of change relevant to urothelial cancer, we have characterised the expression of five genes (p53, MDM2, Rb, E-cadherin, APC) within a panel of human bladder carcinoma cell lines. Using single-strand conformation polymorphism (SSCP) and direct sequencing, p53 mutations were identified in 7/15 (47%) cell lines reflecting events reported in bladder tumours. Immunohistochemical analysis of p53 in cultured cells and in paraffin-embedded sections of xenografts from the cell line panel revealed discordant results. An absence of p53 nuclear staining was associated with an exon 5 mutation in EJ and with multiple p53 mutations found in J82. Two cell lines positive for p53 staining in the absence of detectable mutation displayed overexpression of MDM2 (PSI, HT1197) in Western blot analysis. Loss or aberrant Rb expression was recorded in 5/15 (TCCSUP, SCaBER, 5637, HT1376, J82) cell lines. Absence of E-cadherin was recorded in 5/15 cell lines (TCCSUP, EJ, KK47, UM-UC-3, J82) with loss of alpha-catenin in immunoprecipitated E-cadherin complexes of CUBIII. Western blot analysis of APC revealed a truncated protein in 1/15 (CUBIII) cell lines. The characterisation of oncogenic events within this panel of human bladder carcinoma cell lines establishes a representation of change observed in bladder tumours and better defines the genotypic background in these experimental human cell models of neoplastic progression.
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DOI：
10.1073/pnas.82.11.3849
发表时间：
1985-01-01
影响因子：
11.1
作者：
FUJITA, J;SRIVASTAVA, SK;AARONSON, SA
通讯作者：
AARONSON, SA
DOI：
10.1073/pnas.87.7.2775
发表时间：
1990-04-01
影响因子：
11.1
作者：
HOROWITZ, JM;PARK, SH;WEINBERG, RA
通讯作者：
WEINBERG, RA
影响因子：
56.9
作者：
HOROWITZ, JM;YANDELL, DW;DRYJA, TP
通讯作者：
DRYJA, TP
影响因子：
158.5
作者：
ESRIG, D;ELMAJIAN, D;COTE, RJ
通讯作者：
COTE, RJ
DOI：
10.1093/jnci/86.17.1325
发表时间：
1994-09-07
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
LIANES, P;ORLOW, I;CORDONCARDO, C
通讯作者：
CORDONCARDO, C