Outer membrane vesicles from a mosquito commensal mediate targeted killing of Plasmodium parasites via the phosphatidylcholine scavenging pathway.
Outer membrane vesicles from a mosquito commensal mediate targeted killing of Plasmodium parasites via the phosphatidylcholine scavenging pathway.
复制标题
蚊子共栖外膜囊泡通过磷脂酰胆碱清除途径介导疟原虫的靶向杀伤。
DOI：
10.1038/s41467-023-40887-6
复制
发表时间：
2023-08-24
影响因子：
16.6
通讯作者：
Wang, Sibao
中科院分区：
文献类型：
--
作者：
Gao, Han;Jiang, Yongmao;Wang, Lihua;Wang, Guandong;Hu, Wenqian;Dong, Ling;Wang, Sibao
肠道微生物群是蚊子体内疟原虫感染的关键调节剂，包括抗疟原虫效应蛋白的产生。但是评论者衍生的效应器如何被转移到疟原虫寄生虫中仍然不清楚。本研究表明，一种天然的阻断疟原虫的共生细菌尿溶沙雷菌Su_YN1通过外膜囊泡（omv）将效应脂肪酶AmLip传递给疟原虫。在吸血后，宿主血清强烈诱导Su_YN1释放omv和抗疟效应蛋白AmLip进入蚊子肠道。AmLip首先通过T1SS分泌到细胞外空间，然后优先装载到选择性靶向疟疾寄生虫的omv上，从而导致寄生虫的靶向杀死。值得注意的是，这些血清诱导的OMV含有某些血清来源的脂质，如磷脂酰胆碱，这对于疟原虫通过磷脂酰胆碱清除途径摄取OMV至关重要。这些发现表明，这种肠道共生细菌进化到以细胞外囊泡的形式分泌效应分子，选择性地攻击寄生虫，使蚊子对疟原虫感染产生抗性。肠道菌体衍生的omv作为载体在蚊子微生物群和疟原虫之间跨界交流中的作用的发现，为阻断疟疾传播提供了一种潜在的创新策略。在这里，Gao和他的同事们表明，蚊子的共生细菌尿溶沙雷菌产生囊泡，这些囊泡向疟原虫传递抗疟原虫效应物，并使蚊子对疟原虫的感染产生抵抗力。
The gut microbiota is a crucial modulator of Plasmodium infection in mosquitoes, including the production of anti-Plasmodium effector proteins. But how the commensal-derived effectors are translocated into Plasmodium parasites remains obscure. Here we show that a natural Plasmodium blocking symbiotic bacterium Serratia ureilytica Su_YN1 delivers the effector lipase AmLip to Plasmodium parasites via outer membrane vesicles (OMVs). After a blood meal, host serum strongly induces Su_YN1 to release OMVs and the antimalarial effector protein AmLip into the mosquito gut. AmLip is first secreted into the extracellular space via the T1SS and then preferentially loaded on the OMVs that selectively target the malaria parasite, leading to targeted killing of the parasites. Notably, these serum-induced OMVs incorporate certain serum-derived lipids, such as phosphatidylcholine, which is critical for OMV uptake by Plasmodium via the phosphatidylcholine scavenging pathway. These findings reveal that this gut symbiotic bacterium evolved to deliver secreted effector molecules in the form of extracellular vesicles to selectively attack parasites and render mosquitoes refractory to Plasmodium infection. The discovery of the role of gut commensal-derived OMVs as carriers in cross-kingdom communication between mosquito microbiota and Plasmodium parasites offers a potential innovative strategy for blocking malaria transmission. Here, Gao and colleagues show that the mosquito symbiotic bacterium Serratia ureilytica produces vesicles that deliver an anti-Plasmodium effector to the Plasmodium parasite and render mosquitoes resistant to the infection of malaria parasites.
登录
查看更多内容
影响因子：
7.8
作者：
GRELLIER, P;RIGOMIER, D;SCHREVEL, J
通讯作者：
SCHREVEL, J
细菌活力的降低刺激肠外致病性大肠杆菌 (ExPEC) 细胞质携带的细胞外囊泡 (EV) 的产生
影响因子：
6.7
作者：
通讯作者：
--
影响因子：
16
作者：
Bachurski, Daniel;Schuldner, Maximiliane;von Strandmann, Elke Pogge
通讯作者：
von Strandmann, Elke Pogge
影响因子：
2.8
作者：
Golpayegani, Abdolali;Nodehi, Ramin Nabizadeh;Douraghi, Masoumeh
通讯作者：
Douraghi, Masoumeh
影响因子：
30.3
作者：
Itoe MA;Sampaio JL;Cabal GG;Real E;Zuzarte-Luis V;March S;Bhatia SN;Frischknecht F;Thiele C;Shevchenko A;Mota MM
通讯作者：
Mota MM