Polyamine depletion inhibits the differentiation of L6 myoblast cells.
Polyamine depletion inhibits the differentiation of L6 myoblast cells.
复制标题
多胺消耗抑制 L6 成肌细胞的分化。
DOI：
10.1016/0006-291x(83)90651-4
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Pegg,AE
中科院分区：
文献类型：
--
作者：
Erwin,BG;Ewton,DZ;Florini,JR;Pegg,AE
α-二氟甲基鸟氨酸是一种酶激活的鸟氨酸脱羧酶的不可逆抑制剂，暴露于α-二氟甲基鸟氨酸可以抑制胰岛素诱导的L6成肌细胞分化。通过测量肌酸激酶活性和测定肌管中细胞核的百分比来评估分化。在受刺激的培养物分化之前，腐胺和亚精胺的水平增加，而这些增加被α-二氟甲基鸟氨酸阻断。提供外源性腐胺能够逆转药物的抑制作用。只有在胰岛素刺激后24小时内加入α-二氟甲基鸟氨酸才能观察到抗分化作用。在所使用的实验方案中，α-二氟甲基鸟氨酸在培养接近汇合时加入，对其最终DNA含量没有影响。因此，α-二氟甲基鸟氨酸对成肌细胞分化的影响并非次于对细胞增殖的影响。这些结果表明，多胺可能参与了肌肉细胞分化的调节。
Exposure to α-difluoromethylornithine, an enzyme-activated irreversible inhibitor of ornithine decarboxylase, inhibited the insulin induced differentiation of L6 myoblast cells. Differentiation was assessed by measuring creatine kinase activity and by determining the percentage of nuclei in myotubes. The levels of putrescine and spermidine increased in stimulated cultures prior to their differentiation and these increases were blocked by α-difluoromethylornithine. Provision of exogenous putrescine was able to reverse the inhibitory effect of the drug. The anti-differentiative effect is observed only if α-difluoromethylornithine is added within twenty-four hours of insulin stimulation. In the experimental protocol used, α-difluoromethylornithine was added as the cultures approached confluence and had no effect on their ultimate DNA content. Therefore, the effect of α-difluoromethylornithine on myoblast differentiation is not secondary to an effect on cellular proliferation. These results indicate that polyamines may be involved in the mediation of muscle cell differentiation.