Senescence-associated beta-galactosidase activity expression in aging hippocampal neurons
Senescence-associated beta-galactosidase activity expression in aging hippocampal neurons
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DOI：
10.1016/j.bbrc.2010.05.011
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发表时间：
2010-06-11
影响因子：
3.1
通讯作者：
Fu, Yu-Cai
中科院分区：
文献类型：
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作者：
Geng, Yi-Qun;Guan, Ji-Tian;Fu, Yu-Cai
目的：研究衰老大鼠海马区衰老相关β-半乳糖苷酶(SA-β-GAL)的活性。对6月龄、18月龄和24月龄大鼠海马神经元进行SA-β半乳糖组织化学染色和细胞化学染色。6~18月龄大鼠CA3区锥体细胞SA-β-Gal活性增加一倍(14.57+/-2.74%对31.66+/-14.12%)，24月龄时达50.76+/-14.41%。随着培养海马神经元培养时间的延长，SA-β-Gal的活性也随着时间的延长而增加，在体外培养20天时，95%的细胞SA-β-Gal阳性。有趣的是，在检查的任何年龄，在海马齿状回的神经元中都没有发现SA-β半乳糖阳性细胞，海马齿状回是大脑的一个神经起源区域。SA-β-Gal可作为衰老生物标志物，用于确定晚期衰老大鼠海马CA3区锥体细胞的衰老神经元。(C)2010 Elsevier Inc.保留所有权利。
To investigate the activity of senescence-associated beta-galactosidase (SA-beta-GAL) in the hippocampus of aging rats. Hippocampi of 6-, 18-, and 24-month-old rats were observed by histochemical staining for SA-beta-GAL and cytochemical staining for SA-beta-GAL in cultured hippocampal neurons. The activity of SA-beta-GAL doubled in hippocampal pyramidal cells of the CA3 region in rats between 6 and 18 months (14.57 +/- 2.74% vs. 31.66 +/- 14.12% SA-beta-GAL-positive, respectively), and reached 50.76 +/- 14.41% positive at 24 months. The activity of SA-beta-GAL also increased as a function of time upon prolonged culture of cultured hippocampal neurons with 95% of cells being SA-beta-GAL-positive at 20 days in vitro. Interestingly, no SA-beta-GAL-positive cells were found in neurons of the hippocampal dentate gyrus, a neurogenic region of the brain, at any age examined. SA-beta-GAL can be used as a senescence biomarker in determining senescent neurons in hippocampal pyramidal cells of the CA3 region in advanced aging. (c) 2010 Elsevier Inc. All rights reserved.