Krüppel-like factors 4 and 5 expression and their involvement in differentiation of oral carcinomas.
Krüppel-like factors 4 and 5 expression and their involvement in differentiation of oral carcinomas.
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发表时间：
2015-04
影响因子：
1.4
通讯作者：
Masaki Shibata;T. Chiba;Takanori Matsuoka;Nozomi Mihara;S. Kawashiri;K. Imai
中科院分区：
文献类型：
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作者：
Masaki Shibata;T. Chiba;Takanori Matsuoka;Nozomi Mihara;S. Kawashiri;K. Imai
Krüppel样因子（KLF）4和KLF 5调节上皮细胞的增殖分化平衡，其表达失衡与肿瘤进展有关。然而，很少有人知道在口腔癌的表达和作用。本研究采用免疫组化方法检测了67例乳腺癌中KLF 4和KLF 5的表达及其与癌细胞增殖和分化的关系。KLF 4在角化癌细胞中检测到，KLF 5在非角化癌细胞中检测到。KLF 4在有淋巴结转移的患者中表达下降（P < 0.05），与组织学去分化程度呈平行关系（P = 0.09）。外源性KLF 4过表达的细胞排列成鹅卵石样结构，有桥粒，KLF 5延长细胞，像成纤维细胞一样，没有桥粒。KLF 4抑制纤维连接蛋白的表达，KLF 5下调和降解E-钙粘蛋白。KLF对细胞增殖无明显影响。因此，KLF 4的下调和KLF 5的上调可能通过癌细胞的去分化刺激口腔癌的进展。
Proliferation-differentiation balance of epithelial cells is regulated by Krüppel-like factors (KLF) 4 and 5, and the unbalanced expression relates to carcinoma progression. However, little is known about the expression and role in oral carcinomas. This study examined expression of KLF4 and KLF 5 in the carcinomas by immunohistochemistry (n = 67) and the involvement in proliferation and differentiation of carcinoma cells. KLF4 was detected in keratinizing carcinoma cells and KLF5 in non-keratinizing cells. KLF4 staining declined in the patient with lymph node metastasis (P < 0.05) and in parallel with the histological dedifferentiation (P = 0.09). Exogenous overexpression of KLF4 arranged cells in a cobble-like structure with desmosomes and KLF5 elongated cells like fibroblasts without desmosomes. KLF4 suppressed fibronectin expression, and KLF5 down-regulated and degraded E-cadherin. The proliferation was not affected by KLFs. Thus, down-regulation of KLF4 and up-regulation of KLF5 may stimulate oral carcinoma progression through the dedifferentiation of carcinoma cells.