Effect of stellate ganglionectomy on basal cardiovascular function and responses to β1-adrenoceptor blockade in the rat
Effect of stellate ganglionectomy on basal cardiovascular function and responses to β1-adrenoceptor blockade in the rat
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DOI：
10.1152/ajpheart.00958.2008
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发表时间：
2008-12-01
影响因子：
4.8
通讯作者：
Osborn, John W.
中科院分区：
文献类型：
--
作者：
Yoshimoto, Misa;Wehrwein, Erica A.;Osborn, John W.
星状神经节切除术对大鼠心血管基础功能和β 1受体阻滞反应的影响。Am J Physiol Heart Circ Physiol 295：H2447-H2454，2008。首次发表于2008年10月17日; doi：10.1152/ajpheart.00958.2008。心脏交感神经活动是心脏功能和动脉压的重要短期控制器。研究还表明，心脏交感神经活动的长期增加可能导致高血压、冠状动脉疾病和心力衰竭中的心脏重塑。然而，我们对心交感神经在慢性心血管疾病模型中的作用的理解受到实验方法不足的限制。本研究旨在发展一种外科方法，以外科手术切除大鼠心脏的交感神经，用于长期的心血管研究。我们描述了心脏交感神经去神经对平均动脉压（MAP）和心率（HR）基础水平的影响，以及对长期服用阿替洛尔（一种β（1）-肾上腺素受体拮抗剂）的反应。在4天的基线期后，对大鼠进行双侧星状神经节切除术（SGX; n = 9）或假手术（Sham; n = 8）。在SGX或Sham后7天，对大鼠给予阿替洛尔5天，然后是7天的恢复期。短暂降低后，SGX对基础MAP没有影响，但与基线和Sham大鼠相比，HR降低。阿替洛尔治疗5天后，SGX和Sham大鼠的MAP下降相似。阿替洛尔在Sham大鼠中导致明显的心动过缓，但对SGX大鼠的HR影响可忽略不计。在研究结束时，测量所有心腔中的心脏儿茶酚胺含量，证实了成功的交感神经去神经。本研究证实了双侧SGX是研究心交感神经在心功能调节中的作用的有用方法。此外，这些结果表明，心脏交感神经是相对不重要的维持MAP的基础水平或阿替洛尔在清醒的，不受约束的大鼠的降压反应。
Effect of stellate ganglionectomy on basal cardiovascular function and responses to beta(1)-adrenoceptor blockade in the rat. Am J Physiol Heart Circ Physiol 295: H2447-H2454, 2008. First published October 17, 2008; doi:10.1152/ajpheart.00958.2008.-Cardiac sympathetic nerve activity is an important short-term controller of cardiac function and arterial pressure. Studies also suggest that long-term increases in cardiac sympathetic nerve activity may contribute to hypertension, coronary artery disease, and cardiac remodeling in heart failure. However, our understanding of the role of cardiac sympathetic nerves in chronic models of cardiovascular disease has been limited by inadequate experimental approaches. The present study was conducted to develop a surgical method to surgically denervate the sympathetic nerves of the rat heart for long-term cardiovascular studies. We characterized the effect of cardiac sympathetic denervation on basal levels of mean arterial pressure (MAP) and heart rate (HR) and the responses to a chronic administration of atenolol, a beta(1)-adrenoceptor antagonist. Rats were instrumented with telemetry transmitters for continuous recording of MAP and HR. After a 4-day baseline period, the rats were subjected to bilateral stellate ganglionectomy (SGX; n = 9) or sham surgery (Sham; n = 8). Seven days following SGX or Sham, the rats were administered atenolol for 5 days, followed by a 7-day recovery period. Following a transient decrease, SGX had no effect on basal MAP but decreased HR compared with baseline and Sham rats. Five days of atenolol treatment decreased MAP similarly in SGX and Sham rats. Atenolol resulted in a marked bradycardia in Sham rats but had a neglible effects on HR in SGX rats. The measurement of the content of cardiac catecholamines in all cardiac chambers at the end of the study verified a successful sympathetic denervation. This study confirms that bilateral SGX is a useful method to study the contribution of cardiac sympathetic nerves on the regulation of cardiac function. Moreover, these results suggest that cardiac sympathetic nerves are relatively unimportant in maintaining the basal level of MAP or the depressor response to atenolol in conscious, unrestrained rats.