Effects of inhalation of sevoflurane at different concentrations on TRPV1 in airways of rats at different developmental stages
Effects of inhalation of sevoflurane at different concentrations on TRPV1 in airways of rats at different developmental stages
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吸入不同浓度七氟醚对不同发育阶段大鼠气道TRPV1的影响
DOI：
10.1016/j.lfs.2020.117472
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发表时间：
2020-05-15
期刊：
影响因子：
6.1
通讯作者：
Zhu，Zhaoqiong
中科院分区：
文献类型：
--
作者：
Liu，Dexing;Yuan，Jie;Zhu，Zhaoqiong
目的测定14、21、42日龄大鼠吸入1.5%、2.6%七氟醚后离子通道trpv1、神经肽nka、NKB、降钙素基因相关肽（CGRP）、sp表达的变化。主要方法设计小型室内吸入性麻醉室，14、21、42日龄大鼠分别吸入1.5%、2.6%七氟醚，对照组大鼠吸入载气（1 L/min air +1 L/min O2）。此外，14日龄和21日龄大鼠先用辣椒平预处理，然后分别吸入1.5%和2.6%的七氟醚或载气。Western blot检测肺组织中TRPV1的表达，免疫组化检测气管中NKA、NKB、CGRP、SP的表达。关键发现吸入1.5%七氟醚后，14、21日龄大鼠肺组织中TRPV1的表达较对照组显著升高，且经辣椒平预处理后呈拮抗作用。吸入1.5%七氟醚可显著提高21日龄大鼠气管内NKA、NKB、CGRP和SP的表达，显著提高14日龄大鼠气管内NKB、CGRP和SP的表达。辣椒平预处理可拮抗这些分子的表达。相反，吸入2.6%七氟醚可降低42日龄大鼠气管中NKA和NKB的表达。意义：evoloflurane不上调晚期发育大鼠气道中TRPV1的表达。这种麻醉剂可能对气道有双向作用，在儿科临床麻醉管理中产生相当大的影响。
AimDetermine changes in the expressions of the ion channel—TRPV1—and neuropeptides—NKA, NKB, calcitonin gene-related peptide (CGRP), and SP—in 14-, 21-, and 42-day-old rats after inhaling 1.5% and 2.6% sevoflurane.Main methodsA small in-house inhalation anesthesia chamber was designed to allow 14-, 21-, and 42-day-old rats inhale 1.5% and 2.6% sevoflurane, and rats in the control group inhaled carrier gas(1 L/min air +1 L/min O2). In addition, 14- and 21-day-old rats were pretreated with capsazepine, followed by inhalation of 1.5% and 2.6% sevoflurane or the carrier gas. The expression of TRPV1 in lung tissues was detected by Western blotting, whereas the expressions of NKA, NKB, CGRP, and SP in the trachea were detected by immunohistochemistry.Key findingsAfter inhalation of 1.5% sevoflurane, the expression of TRPV1 in the lung tissues of 14- and 21-day-old rats was significantly increased compared with that in the control group, which was antagonized by capsazepine pretreatment. Moreover, inhalation of 1.5% sevoflurane markedly increased the expressions of NKA, NKB, CGRP, and SP in the trachea of 21-day-old rats and of NKB, CGRP, and SP in the trachea of 14-day-old rats. The expressions of these molecules were antagonized by capsazepine pretreatment. Conversely, inhalation of 2.6% sevoflurane decreased the expressions of NKA and NKB in the trachea of 42-day-old rats.SignificanceSevoflurane did not upregulate the expression of TRPV1 in the airways of late-developing rats. This anesthetic may have a two-way effect on airways, resulting in considerable effects in pediatric clinical anesthesia management.