Construction and Application of a Novel Impedance Sensor for Siderophore
Construction and Application of a Novel Impedance Sensor for Siderophore
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DOI：
10.1080/00032719.2014.938341
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发表时间：
2015-01
影响因子：
2
通讯作者：
F. He;Danyang Yan
中科院分区：
文献类型：
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作者：
F. He;Danyang Yan
铁载体是一种潜在的病原菌诊断生物标志物。在这项研究中，铁载体阻抗传感器构建的基础上形成的铁载体-氯化高铁血红素复合物制备的氯化高铁血红素修饰的叉指阵列微电极。铁载体与氯化血红素在电极表面形成络合物。由于氯化血红素的电导率高于铁载体的电导率，因此配合物的形成导致了电极表面电导率的降低。该传感器的阻抗变化与铁载体浓度在1 nM ~ 100 µM之间呈线性关系，检测限为0.86 nM。检测时间小于15 min，用于介质分析，回收率在86.7%~ 102.8%之间。与传统的铁载体检测方法相比，该方法具有快速、灵敏的特点，在病原菌检测中具有潜在的应用价值。
Siderophore is a potential biomarker for pathogen diagnosis. In this study, a siderophore impedance sensor was constructed based on the formation of a siderophore-hemin complex prepared by modification of hemin on an interdigitated array microelectrode. Siderophore reacted with hemin and formed a complex on the electrode surface. As the conductivity of hemin was higher than that of siderophore, the formation of the complex led to decrease of conductivity on electrode surface which was measured by electrochemical impedance spectroscopy. The impedance change of constructed sensor was linear with the concentration of siderophore between 1 nM and 100 µM, with a limit of detection of 0.86 nM. The detection time was less than 15 min. When the sensor was used to analyze medium, the recovery was between 86.7% and 102.8%. Compared with conventional methods for siderophore, this approach is faster, more sensitive, and has potential application for the determination of pathogens.