A time-resolved spectral study of the K and KL intermediates of bacteriorhodopsin.
A time-resolved spectral study of the K and KL intermediates of bacteriorhodopsin.
复制标题
细菌视紫红质 K 和 KL 中间体的时间分辨光谱研究。
DOI：
10.1016/s0006-3495(88)83124-2
复制
发表时间：
1988
影响因子：
3.4
通讯作者：
Kliger,DS
中科院分区：
文献类型：
--
作者：
Milder,SJ;Kliger,DS
报道了细菌视紫红质（BR）在完整膜中光解产物的纳秒时间分辨吸收光谱测量。在室温下，在流体溶液中，激发后10 ns观察到单个中间体（KL）。光谱和动力学结果均与KL中间体通过单一一级反应转化为L中间体一致。所观察到的温度依赖性速率具有阿克里尼乌斯参数：Ea=10.5 kcal/mol，A=5 x 10（13）s-1。还观察到KL中间体的前体。其光谱特征与已报道的K中间体一致。目前的数据是一致的，在BR光循环的K，KL和L在室温下的流体溶液中的线性序列。在这里和其他地方所描述的K中间体的光谱特性的差异进行了讨论，在视网膜部分周围的微环境的差异和影响，这可能对构象的发色团。
Nanosecond time-resolved absorption measurements on the photolysis products of bacteriorhodopsin (BR) in intact membranes are reported. At room temperature in fluid solution a single intermediate (KL) is seen 10 ns after excitation. Both spectral and kinetic results are consistent with the KL intermediate converting to the L intermediate by a single first order reaction. The observed temperature-dependent rate has the Arrhenius parameters: Ea=10.5 kcal/mol, A=5 x 10(13) s-1. The precursor to the KL intermediate is also observed. Its spectral character is consistent with the K intermediate which has been previously reported. The current data is consistent with a linear sequence in the BR photocycle for K, KL, and L in room temperature fluid solution. Differences in the spectral characteristics of the K intermediates described here and elsewhere are discussed in terms of differences in the microenvironment around the retinal moiety and the affect this may have on the conformation of the chromophore.