EFFECTS OF DEXAMETHASONE ON STEROIDOGENESIS IN LEYDIG-CELLS FROM RATS OF DIFFERENT AGES
EFFECTS OF DEXAMETHASONE ON STEROIDOGENESIS IN LEYDIG-CELLS FROM RATS OF DIFFERENT AGES
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DOI：
10.1016/0960-0760(95)00058-8
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发表时间：
1995-07-01
影响因子：
4.1
通讯作者：
VIND, C
中科院分区：
文献类型：
--
作者：
AGULAR, BM;VIND, C
研究了0.1 μ M地塞米松对3、5、7和10周龄大鼠Leydig细胞细胞色素P450含量、3 β-羟基类固醇脱氢酶（3 β-HSD）活性以及基础和LH诱导的睾酮产生的影响。地塞米松处理3周龄大鼠睾丸间质细胞22 h后，细胞色素P450含量增加，而3 β-HSD活性降低。5周龄大鼠睾丸间质细胞的细胞色素P450含量在培养3和22 h后增加，而3 β-HSD活性在处理22和44 h后降低。7周龄大鼠细胞色素P450含量在培养3 h后升高，而3 β-HSD活性在培养22和44 h后降低。地塞米松处理3 h后，10周龄大鼠睾丸间质细胞的细胞色素P450含量增加。处理44 h后3 β-HSD活性降低。在3周龄和5周龄大鼠的Leydig细胞中，地塞米松在治疗22小时后降低基础睾酮产生，但在44小时后不降低，并且不影响LH诱导的睾酮产生。7周龄大鼠睾丸间质细胞用地塞米松处理22和44 h后，基础和LH诱导的睾酮产生减少。基础睾酮的生产是不受地塞米松在大鼠10周龄，而LH诱导的睾酮生产后44小时的治疗减少。地塞米松对睾酮分泌的影响在发育过程中发生变化，在青春期前和青春期大鼠的Leydig细胞中观察到对基础睾酮分泌的短暂早期影响。这些数据表明，地塞米松对间质细胞的影响不同，这取决于大鼠的年龄，老年大鼠比年轻大鼠更敏感。
The effects of 0.1 mu M dexamethasone on cytochrome P450 content, 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) activity, and basal and LH-induced testosterone production of Leydig cells from rats 3, 5, 7 and 10 weeks old were examined. The cytochrome P450 content of Leydig cells from rats 3 weeks old was increased by treatment with dexamethasone for 22 h, while 3 beta-HSD activity was decreased. The cytochrome P450 content of Leydig cells from rats 5 weeks old was increased after 3 and 22 h of culture, while 3 beta-HSD activity was decreased after 22 and 44 h of treatment. The cytochrome P450 content of rats 7 weeks old was increased after 3 h of culture, while 3 beta-HSD activity was decreased after 22 and 44 h of culture. Leydig cells from rats 10 weeks old showed increased cytochrome P450 content upon dexamethasone treatment after 3 h. The activity of 3 beta-HSD was decreased after 44 h of treatment. In Leydig cells from rats 3 and 5 weeks old, dexamethasone decreased basal testosterone production after 22 h of treatment, but not after 44 h, and did not affect LH-induced testosterone production. Leydig cells from rats 7 weeks old showed decreased basal and LH-induced testosterone production, when treated with dexamethasone for 22 and 44 h. Basal testosterone production was unaffected by dexamethasone in rats 10 weeks old, while LH-induced testosterone production was decreased after 44 h of treatment. The effect of dexamethasone on testosterone secretion changed during development, as a transient, early effect on basal testosterone secretion was observed in Leydig cells from prepubertal and pubertal rats. These data suggest that dexamethasone affects Leydig cells differently, depending on the age of the rat, the older rats being more sensitive than the younger rats.