Adenosine Release from Isolated Rat Adipocytes: Influence of Fat Cell Concentration and Cell Size 1
Adenosine Release from Isolated Rat Adipocytes: Influence of Fat Cell Concentration and Cell Size 1
复制标题
离体大鼠脂肪细胞的腺苷释放：脂肪细胞浓度和细胞大小的影响 1
DOI：
10.3181/00379727-182-42303
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发表时间：
1986
影响因子：
--
通讯作者：
Z. Israili
中科院分区：
文献类型：
--
作者：
M. Capogrossi;Mario Di Girolamo;H. Zimring;D. Crandall;A. Francendese;Z. Israili
摘要本文研究了离体大鼠脂肪细胞释放腺苷与脂肪细胞大小和浓度的关系。在含有6 mM葡萄糖的Krebs-Ringer碳酸氢盐-白蛋白培养基中于37°C孵育60分钟。加入2′-脱氧coformycin以抑制内源性腺苷脱氨酶活性（在0.8 μM抑制剂浓度下达到最大抑制）。数据表明：（a）在第一个和第二个30分钟孵育期，释放到培养基中的腺苷量相似;（B）增加脂肪细胞浓度显著抑制腺苷释放;和（c）大脂肪细胞（体积> 300 pl）释放显著更多的腺苷当以≥ 350，000个细胞/ml的浓度孵育时，与较小的脂肪细胞（体积< 180 μ l）相比，高于此细胞浓度，腺苷释放和细胞大小之间的差异没有被注意到。腺苷释放的大鼠脂肪细胞似乎是一个精确的调节过程，这是exquisitly敏感的脂肪细胞在孵育培养基中的数量，并在一定程度上，脂肪细胞的大小。
Abstract The release of adenosine by isolated rat adipocytes into the incubation medium was studied in relation to fat cell size and concentration. Incubations were carried out for 60 min at 37°C in Krebs-Ringer bicarbonate-albumin medium containing 6 mM glucose. 2′-Deoxycoformycin was added to inhibit endogenous adenosine deaminase activity (maximal supression was achieved at 0.8 μM concentration of the inhibitor). The data show that (a) the amount of adenosine released into the medium was similar for the first and second 30-min incubation periods; (b) increasing adipocyte concentration markedly inhibited adenosine release; and (c) large fat cells (volume > 300 pl) released significantly more adenosine (per fat cell) into the medium than smaller fat cells (volume < 180 pl) when incubated at concentrations of ≥ 350,000 cells/ml. Above this cell concentration, differences between adenosine release and cell size were not noted. Adenosine release by isolated rat adipocytes appears to be a precisely regulated process which is exquisitly sensitive to the number of fat cells in the incubation medium and, to a certain extent, to the adipocyte size.