COMPUTATIONAL ANALYSIS OF STRUCTURAL AND ENERGETIC CONSEQUENCES OF DNA METHYLATION
COMPUTATIONAL ANALYSIS OF STRUCTURAL AND ENERGETIC CONSEQUENCES OF DNA METHYLATION
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DOI：
10.1093/carcin/10.6.1131
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发表时间：
1989-06-01
期刊：
影响因子：
4.7
通讯作者：
BAYLIN, SB
中科院分区：
文献类型：
--
作者：
HAUSHEER, FH;RAO, SN;BAYLIN, SB
真核生物中胞嘧啶甲基化与转录或染色质结构形成有关的构象和能量后果还没有很好地理解。在不同的离子条件下，通过理论方法研究了生物相关来源的甲基化和未甲基化DNA序列的结构，并证明胞嘧啶甲基化在DNA中产生空间、疏水、能量、构象和静电改变的局部模式。这些发现表明这种修饰可能如何影响蛋白质-DNA相互作用并支持当前的假设。结果揭示了以前未被认识到的胞嘧啶甲基化的潜在影响，其可以通过改变转录事件、组蛋白结合、染色体稳定性和细胞分化来严重影响正常和肿瘤细胞过程。
The conformational and energetic consequences of cytosine methylation in eukaryotes related to transcription or formation of chromatin structure are not well understood. Structures of methylated and unmethylated DNA sequences from biologically relevant sources were studied by theoretical methods under different ionic conditions and demonstrate that cytosine methylation produces a localized pattern of steric, hydrophobic, energic, conformational and electrostatic alterations in DNA. These findings suggest how this modification may influence protein-DNA interactions and support current hypotheses. The results reveal previously unrecognized potential effects of cytosine methylation which could critically affect normal and neoplastic cellular processes by altering transcriptional events, histone binding, chromosomal stability and cellular differentiation.