siRNA targeting Vaccinia virus double-stranded RNA binding protein [E3L] exerts potent antiviral effects
siRNA targeting Vaccinia virus double-stranded RNA binding protein [E3L] exerts potent antiviral effects
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DOI：
10.1016/j.virol.2006.01.013
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发表时间：
2006-05-10
期刊：
影响因子：
3.7
通讯作者：
Pomerantz, RJ
中科院分区：
文献类型：
--
作者：
Dave, RS;McGettigan, JP;Pomerantz, RJ
牛痘病毒基因E3 L编码双链RNA [dsRNA]结合蛋白。我们假设，由于dsRNA在触发宿主先天性抗病毒反应中的关键性质，E3 L特异性小干扰RNA [siRNA]应该是对抗痘病毒的有效抗病毒剂，而牛痘病毒是合适的替代物。在这项研究中，我们利用了两种人类细胞类型，即HeLa和293 T，一种对干扰素[IFN]-β有反应，另一种分别产生并对IFN-β有反应。抗病毒作用在HeLa和293 T细胞中同样稳健。然而，在293 T细胞的情况下，当IFN-β在这些细胞中被激活时，观察到几个不同的特征。与用E3 L特异性siRNA转染的HeLa和293 T细胞中的对照感染相比，牛痘病毒复制分别被抑制97%和98%。这些研究证明了E3 L特异性SiRNA作为天花和相关痘病毒的有效抗病毒剂的实用性。(c)2006年爱思唯尔公司All rights reserved.
The Vaccinia virus gene, E3L, encodes a double-stranded RNA [dsRNA]-binding protein. We hypothesized that, owing to the critical nature of dsRNA in triggering host innate antiviral responses, E3L-specific small-interfering RNAs [siRNAs] should be effective antiviral agents against pox viruses, for which Vaccinia virus is an appropriate surrogate. In this study, we have utilized two human cell types, namely, HeLa and 293T, one which responds to interferon [IFN]-beta and the other produces and responds to IFN-beta respectively. The antiviral effects were equally robust in HeLa and 293T cells. However, in the case of 293T cells, several distinct features were observed, when IFN-beta is activated in these cells. Vaccinia virus replication was inhibited by 97% and 98% as compared to control infection in HeLa and 293T cells transfected with E3L-specific siRNAs, respectively. These Studies demonstrate the utility of E3L-specific siRNAs as potent antiviral agents for small pox and related pox viruses. (c) 2006 Elsevier Inc. All rights reserved.