Brefeldin A inhibits vaccinia virus envelopment but does not prevent normal processing and localization of the putative envelopment receptor P37.
Brefeldin A inhibits vaccinia virus envelopment but does not prevent normal processing and localization of the putative envelopment receptor P37.
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Brefeldin A 抑制牛痘病毒包膜，但不会阻止假定的包膜受体 P37 的正常加工和定位。
DOI：
10.1099/0022-1317-76-1-103
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hruby,DE
中科院分区：
文献类型：
--
作者：
Ulaeto,D;Grosenbach,D;Hruby,DE
研究发现，真菌代谢物 Brefeldin A 可以抑制痘苗病毒感染性有包膜形式的产生，但感染性细胞内形式的产生并未受到影响。子代病毒粒子的电子显微镜分析和氯化铯密度离心表明药物阻断不是由于细胞内包膜病毒粒子的保留和积累。对痘苗病毒候选包膜受体（病毒蛋白 P37）的生化分析表明，该药物对该蛋白的棕榈酰化没有明显影响，并且不会阻止或改变其与细胞内膜的关联。这表明P37实际上可能不是痘苗病毒包膜细胞内膜上的受体，并且留下了该分子在包膜过程中发挥什么功能的问题。
The fungal metabolite Brefeldin A was found to inhibit the production of the infectious enveloped form of vaccinia virus, although production of the infectious intracellular form was not affected. Electron microscopic analysis and caesium chloride density centrifugation of progeny virions indicates that the drug block is not due to retention and accumulation of enveloped virions within the cell. Biochemical analysis of the candidate envelopment receptor for vaccinia virus, viral protein P37, shows that the drug has no discernible effect on palmitylation of this protein and does not prevent or alter its association with intracellular membranes. This suggests that P37 may not in fact be the receptor on intracellular membranes for vaccinia virus envelopment, and leaves open the question of what function this molecule performs in the envelopment process.