ON THE NATURE OF CALCIUM-ION BINDING BETWEEN PHOSPHATIDYLSERINE LAMELLAE
ON THE NATURE OF CALCIUM-ION BINDING BETWEEN PHOSPHATIDYLSERINE LAMELLAE
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DOI：
10.1021/bi00367a071
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发表时间：
1986-09-23
期刊：
影响因子：
2.9
通讯作者：
FEIGENSON, GW
中科院分区：
文献类型：
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作者：
FEIGENSON, GW
磷脂酰丝氨酸（PS）片层之间的Ca2+结合产生了一个组成为Ca(PS)2的相。当水中Ca2+、水合PS和Ca(PS)2共存于平衡状态时，水中Ca2+浓度不变。在Ca2+浓度低于这个临界值，没有Ca2+结合到PS检测。超过这个值，Ca2+结合到PS形成Ca(PS)2。不变Ca2+浓度为0.14 μ。M为棕榈酰磷脂酰丝氨酸（POPs）， 3.0。M为二油基磷脂酰丝氨酸（DOPS）。对于来自牛脑的混合酰基链PS (BBPS)， Ca2+浓度范围为0.25至0.7 μ m。在温度和压力恒定的情况下，水、Ca2+和PS三组分体系的相规律描述了所观察到的相行为。为了使Ca2+结合在PS片层之间形成Ca(PS)2相，水中Ca2+浓度必须过饱和。平衡Ca2+浓度是通过Ca2+螯合剂溶解Ca(PS)2来确定的。
Ca2+ binding between phosphatidylserine (PS) lamellae gives rise to a phase with the composition Ca(PS)2. When aqueous Ca2+, hydrated PS, and Ca(PS)2 coexist at equilibrium, the aqueous Ca2+ concentration is invariant. At Ca2+ concentrations below this critical value, no binding of Ca2+ to PS is detected. Above this value, Ca2+ binds to PS to form Ca(PS)2. The invariant Ca2+ concentration is 0.14 .mu.M for palmitoyloleoylphosphatidylserine (POPs) and 3.0 .mu.M for dioleoylphosphatidylserine (DOPS). For the mixed acyl chain PS derived from bovine brain (BBPS) this Ca2+ concentration ranges from 0.25 to 0.7 .mu.M. The observed phase behavior is described by the phase rule for the three-component system of water, Ca2+, and PS, with temperature and pressure constant. In order for Ca2+ to bind between PS lamellae to form the Ca(PS)2 phase, the aqueous Ca2+ concentration must be supersaturated. The equilibrium Ca2+ concentration is determined by dissolving Ca(PS)2 by use of Ca2+ chelators.