Proteomics: challenges, techniques and possibilities to overcome biological sample complexity.
Proteomics: challenges, techniques and possibilities to overcome biological sample complexity.
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DOI：
10.4061/2009/239204
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发表时间：
2009-12-08
期刊：
影响因子：
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通讯作者：
Qian, Pei-Yuan
中科院分区：
文献类型：
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作者：
Chandramouli, Kondethimmanahalli;Qian, Pei-Yuan
蛋白质组学是对复杂生物样品中蛋白质结构和功能的大规模研究。这种方法对于理解生物体的复杂性质具有潜在的价值。目前的蛋白质组学工具允许大规模，高通量分析的检测，鉴定和功能研究的蛋白质组。蛋白质分级分离和标记技术的进步已经改善了蛋白质鉴定，包括最不丰富的蛋白质。此外，蛋白质组学还得到了翻译后修饰分析和不同蛋白质组定量比较技术的补充。然而，蛋白质组学研究的主要限制仍然是生物结构和生理过程的复杂性，使得探索的道路充满了各种困难和陷阱。通过新技术获取的数据量对数据处理和分析提出了新的挑战。本文简要介绍了目前可用的蛋白质组学技术及其应用，然后详细描述了优势和技术挑战。提出了一些解决技术难题的方法。
Proteomics is the large-scale study of the structure and function of proteins in complex biological sample. Such an approach has the potential value to understand the complex nature of the organism. Current proteomic tools allow large-scale, high-throughput analyses for the detection, identification, and functional investigation of proteome. Advances in protein fractionation and labeling techniques have improved protein identification to include the least abundant proteins. In addition, proteomics has been complemented by the analysis of posttranslational modifications and techniques for the quantitative comparison of different proteomes. However, the major limitation of proteomic investigations remains the complexity of biological structures and physiological processes, rendering the path of exploration paved with various difficulties and pitfalls. The quantity of data that is acquired with new techniques places new challenges on data processing and analysis. This article provides a brief overview of currently available proteomic techniques and their applications, followed by detailed description of advantages and technical challenges. Some solutions to circumvent technical difficulties are proposed.