Sex-dependent effects of microcystin-LR on hypothalamic-pituitary-gonad axis and gametogenesis of adult zebrafish.
Sex-dependent effects of microcystin-LR on hypothalamic-pituitary-gonad axis and gametogenesis of adult zebrafish.
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微囊藻蛋白LR对下丘脑 - 垂体轴轴的性别依赖性作用和成年斑马鱼的配子发生。
DOI：
10.1038/srep22819
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发表时间：
2016-03-10
影响因子：
4.6
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Liu W;Chen C;Chen L;Wang L;Li J;Chen Y;Jin J;Kawan A;Zhang X
虽然有报道称微囊藻毒素对鱼类具有生殖毒性，且具有性别依赖性，但其潜在的机制却很少被研究。在本研究中，斑马鱼分别暴露于1、5和20 μg/L的MC-LR中30 d。所有处理过的雄性的性腺-躯体指数都下降了。所有治疗组女性血清中17β-雌二醇（E2）、睾酮(T)、11-酮睾酮（11-KT）和促卵泡激素（FSH）水平均升高，而所有治疗组男性血清中T、FSH和黄体生成素（LH）水平均发生变化。组织形态学观察表明，MC-LR暴露明显延缓了卵子和精子的发生。下丘脑-垂体-性腺（HPG）轴上22个基因的转录变化表现出性别特异性反应，并通过主成分分析（PCA）评估了基因转录与配子体发生的关系。PC1的主要贡献因子（gnrh2、gnrhr3、ar、lhr、hmgra、hmgrb和cyp19a）与卵黄后卵母细胞数量呈正相关，PC1 （gnrh2、lhβ、erβ、fshr、cyp11a和17βhsd）与精子数量呈正相关。17βHSD和CYP19a蛋白水平在雌性和雄性中均受到影响。综上所述，本研究首次探讨了微囊藻毒素对鱼类繁殖的性别依赖性，揭示了MC-LR斑马鱼配子体发生相关的一些重要分子生物标志物。
While microcystins (MCs) have been reported to exert reproductive toxicity on fish with a sex-dependent effect, the underlying mechanism has been rarely investigated. In the present study, zebrafish were exposed to 1, 5 and 20 μg/L MC-LR for 30 d. The gonad-somatic index declined in all treated males. 17β-estradiol (E2), testosterone (T), 11-keto testosterone (11-KT) and follicle-stimulating hormone (FSH) levels increased in serum from all treated females, while T, FSH and luteinizing hormone (LH) levels changed in all treated males. Histomorphological observation showed that MC-LR exposure evidently retarded oogenesis and spermatogenesis. Transcriptional changes of 22 genes of the hypothalamic-pituitary-gonad (HPG) axis exhibited sex-specific responses, and the relationship between gene transcriptions and gametogenesis was evaluated by principle component analysis (PCA). Major contributors to PC1 (gnrh2, gnrhr3, ar, lhr, hmgra, hmgrb and cyp19a) were positively correlated with the number of post-vitellogenic oocytes, while PC1 (gnrh2, lhβ, erβ, fshr, cyp11a and 17βhsd) were positively correlated with the number of spermatozoa. The protein levels of 17βHSD and CYP19a were affected in both females and males. In conclusion, this study first investigated the sex-dependent effects of microcystins on fish reproduction and revealed some important molecular biomarkers related to gametogenesis in zebrafish suffered from MC-LR.