Induction of hepatic antioxidants in freshwater catfish (Channa punctatus Bloch) is a biomarker of paper mill effluent exposure
Induction of hepatic antioxidants in freshwater catfish (Channa punctatus Bloch) is a biomarker of paper mill effluent exposure
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DOI：
10.1016/s0304-4165(00)00098-2
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发表时间：
2000-09-01
影响因子：
3
通讯作者：
Raisuddin, S
中科院分区：
文献类型：
--
作者：
Ahmad, I;Hamid, T;Raisuddin, S
酶和非酶抗氧化剂作为一种重要的生物防御环境氧化应激。尽管鱼类经常暴露在包括氧化剂在内的无数环境压力中，但有关鱼类抗氧化防御的信息却很少。因此，本研究评估了抗氧化酶的活性，即，谷胱甘肽过氧化物酶、过氧化氢酶和谷胱甘肽S-转移酶以及非酶抗氧化剂，谷胱甘肽和金属硫蛋白在淡水鱼Channa punctatus（Bloch）的各种组织，在短期和长期暴露于造纸厂废水。将鱼暴露于浓度为1.0%（v/v）的废水中15，30，60和90天。暴露引起谷胱甘肽水平（P < 0.001）、谷胱甘肽过氧化物酶活性（P < 0.05 ~ P < 0.001）、谷胱甘肽S-转移酶活性（P < 0.001）呈时间依赖性升高，而过氧化氢酶活性在初始时略有下降（P < 0.01 ~ P < 0.001）。暴露60天后，在肝脏中诱导金属硫蛋白。检测到两种金属硫蛋白亚型。过氧化氢酶活性也增加60天后。鳃和肾的抗氧化模式不同，表明肝脏比鳃和肾更能抵抗氧化损伤。我们的研究结果表明，污染诱导的适应性反应在鱼类。此外，酶和非酶组织抗氧化剂的水平可以作为替代指标暴露于氧化剂污染物的鱼。(C)2000 Elsevier Science B. V.保留所有权利。
Enzymatic and non-enzymatic antioxidants serve as an important biological defense against environmental oxidative stress. Information on antioxidant defense in fish is meager despite that fish are constantly exposed to a myriad of environmental stress including the oxidants. This study, therefore, assesses the activities of antioxidant enzymes viz., glutathione peroxidase, catalase and glutathione S-transferase and the non-enzymatic antioxidants viz., glutathione and metallothionein in Various tissues of freshwater fish Channa punctatus (Bloch), in response to short-term and long-term exposures to paper mill effluent. The fish were exposed to the effluent at a concentration of 1.0% (v/v) for 15, 30, 60 and 90 days. The exposure caused a time-dependent increase in glutathione level (P < 0.001), activities of glutathione peroxidase (P < 0.05 to P < 0.001), glutathione S-transferase (P < 0.001) and a marginal initial decrease in catalase activity in the liver (P < 0.01 to P < 0.001). Metallothionein was induced in liver after 60 days of exposure. Two isoforms of metallothionein were detected. Catalase activity also increased 60 days afterwards. Antioxidant pattern was different in gill and kidney showing that liver was more resistant to oxidative damage as compared to gills and kidney. Our results demonstrate a pollutant-induced adaptive response in fish. In addition, levels of enzymatic and non-enzymatic tissue antioxidants may serve as surrogate markers of exposure to oxidant pollutants in fish. (C) 2000 Elsevier Science B.V. All rights reserved.