TRANSPOSON MUTAGENESIS OF BACULOVIRUSES - ANALYSIS OF TRICHOPLUSIA-NI TRANSPOSON IFP2 INSERTIONS WITHIN THE FP-LOCUS OF NUCLEAR POLYHEDROSIS VIRUSES
TRANSPOSON MUTAGENESIS OF BACULOVIRUSES - ANALYSIS OF TRICHOPLUSIA-NI TRANSPOSON IFP2 INSERTIONS WITHIN THE FP-LOCUS OF NUCLEAR POLYHEDROSIS VIRUSES
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DOI：
10.1016/0042-6822(89)90117-7
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发表时间：
1989-09-01
期刊：
影响因子：
3.7
通讯作者：
FRASER, MJ
中科院分区：
文献类型：
--
作者：
CARY, LC;GOEBEL, M;FRASER, MJ
Trichoplusia ni的转座IFP 2元件最初是作为大蜡螟或苜蓿银纹夜蛾核型多角体病毒（NPVs）的自发FP突变体中的宿主DNA插入而分离的。对来自五个独立分离的FP突变体的IFP 2插入的末端进行测序。在所有情况下，IFP 2的侧翼是13 bp的末端反向重复序列，并具有相对于元件末端不对称定位的长度为19 bp的额外反向重复序列。IFP 2插入到病毒基因组中总是产生四核苷酸靶位点TTAA的重复。有一个明显的偏好内插入一个12 bp的A+ T-丰富的不完善的回文序列周围的目标网站。三个独立的IFP 2元件的序列分析揭示了一个2.475 kb的内部结构域包含一个RNA聚合酶II启动子区和两个大的开放阅读框。位于转录物5“末端的IFP 2特异性mRNA的引物延伸分析。该元件存在于从T. ni细胞系TN-368和TN-5 B1的DNA中，但在从TN-R2细胞系或我们的实验室T. ni幼虫，表明IFP 2最近被引入T. ni基因组。
The transposable IFP2 element of Trichoplusia ni was originally isolated as a host DNA insertion in spontaneous FP mutants of Galleria mellonella or Autographa californica nuclear polyhedrosis viruses (NPVs). The termini of IFP2 insertions from five independently isolated FP mutants were sequenced. In all cases IFP2 is flanked by 13-bp terminal inverted repeats and has additional inverted repeats of 19 bp in length located asymmetrically with respect to the ends of the elements. Insertion of IFP2 into the viral genome always generated a duplication of the tetranucleotide target site, TTAA. There was an apparent preference for insertion within a 12-bp A+T-rich imperfect palindromic sequence surrounding the target site. Sequence analysis of three independent IFP2 elements revealed an internal domain of 2.475 kb containing an RNA polymerase II promoter region and two large open reading frames. Primer extension analysis of IFP2-specific mRNA positioned the 5'' terminus of the transcript. The element is present in DNA isolated from T. ni cell lines TN-368 and TN-5B1, but is not apparent in DNAs isolated from the TN-R2 cell line or our laboratory colony of T. ni larvae, suggesting IFP2 was recently introduced into the T. ni genome.