Ultrashort laser based two-photon phase-resolved fluorescence lifetime measurement method
Ultrashort laser based two-photon phase-resolved fluorescence lifetime measurement method
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DOI：
10.1088/2050-6120/ab71c2
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发表时间：
2020-01
影响因子：
3.2
通讯作者：
Hafizah Halip;Yuto Yoshimura;W. Inami;Y. Kawata
中科院分区：
文献类型：
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作者：
Hafizah Halip;Yuto Yoshimura;W. Inami;Y. Kawata
提出了一种基于超短脉冲激光的双光子相位分辨荧光寿命测量方法。所提出的方法还涉及使用锁定放大器来控制参考信号和荧光信号之间的相位差，从而便于使用用于确定荧光寿命的替代方法。使用罗丹明B和细胞热探针作为样品进行本研究中测量的荧光寿命的验证。在这项研究中，我们假设在所有情况下，荧光衰减是单指数的。罗丹明B的平均荧光寿命为2.15 ns，而细胞热探针在33.79-37.2 °C的温度范围内的温度灵敏度为1.39 ns C−1。这些结果验证了所提出的方法的可行性，使用一个简单的激光配置精确测量荧光寿命。
This paper presents a two-photon phase-resolved fluorescence-lifetime measurement method based on the use of an ultrashort pulse laser. The proposed method also involves the use of a lock-in amplifier to control the phase difference between the reference and fluorescence signals, thereby facilitating the use of an alternative method for determining fluorescence lifetimes. Verification of the fluorescence lifetimes as measured in this study was performed using rhodamine B and a cellular thermoprobe as samples. In this study, we assume that the fluorescence decay was monoexponential in all cases. Rhodamine B was observed to exhibit an average fluorescence lifetime of 2.15 ns, whereas a temperature sensitivity of 1.39 ns C−1 over a temperature range of 33.79–37.2 °C was demonstrated for the cellular thermoprobe. These results validate the feasibility of the proposed method for accurate measurement of fluorescence lifetimes using a simple laser configuration.