Synthesis and properties of oligonucleotides modified with 2'-O-(2-carboxyethyl)nucleotides and their carbamoyl derivatives.
Synthesis and properties of oligonucleotides modified with 2'-O-(2-carboxyethyl)nucleotides and their carbamoyl derivatives.
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2-O-(2-羧乙基)核苷酸及其氨基甲酰衍生物修饰的寡核苷酸的合成和性质。
DOI：
10.1039/c4ob01260g
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Sekine M
中科院分区：
文献类型：
--
作者：
Yamada T;Masaki Y;Okaniwa N;Kanamori T;Ohkubo A;Tsunoda H;Seio K;Sekine M
合成了具有4种2′- o修饰尿苷衍生物的2′- o -甲基低核糖核苷酸。通过光谱分析和分子动力学模拟研究了它们的双相稳定性、水合行为和抗外切酶性。因此，用2-氨基甲酰乙基或2-（n -甲基氨基甲酰）乙基对尿苷残基进行2 ' - o修饰，可以显著提高其抗外切酶能力，而不会失去双相稳定性。
2′-O-Methyl oligoribonucleotides with four kinds of 2′-O-modified uridine derivatives were synthesised. Their duplex stability, hydration behavior and exonuclease resistance were studied by spectroscopic analyses and molecular dynamics simulations. Consequently, 2′-O-modification of the uridine residue with 2-carbamoylethyl or 2-(N-methylcarbamoyl)ethyl groups resulted in a significant improvement of the exonuclease resistance without the loss of duplex stability.