A microsomal (cytochrome P-450)-linked lauric-acid-monooxygenase from aged Jerusalem-artichoke-tuber tissues.
A microsomal (cytochrome P-450)-linked lauric-acid-monooxygenase from aged Jerusalem-artichoke-tuber tissues.
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来自老化的菊芋块茎组织的微粒体（细胞色素 P-450）连接的月桂酸单加氧酶。
DOI：
10.1111/j.1432-1033.1978.tb12586.x
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
F. Durst,
中科院分区：
文献类型：
--
作者：
J. Salaün;I. Benveniste;D. Reichhart;F. Durst,
耶路撒冷（Helianthus tuberosus L.）块茎。根据甲基三甲基硅衍生物的质谱裂解谱，确定了三种反应产物：10-羟基月桂酸、9-羟基月桂酸和8-羟基月桂酸。酶活性位于微粒体部分，该部分还携带细胞色素P-450和NADPH细胞色素-c还原酶。该酶对月桂酸的表观Km为0.97 μ M。月桂酸单加氧作用依赖于O2，并被CO抑制，后者的作用是光可逆的。NADPH是优选的电子供体，尽管观察到可观的NADH持续活性。NADPH细胞色素c还原酶参与电子转移，如NADP+和氧化细胞色素c对月桂酸单加氧的抑制作用所证明的。因此，催化月桂酸氧化的酶在耶路撒冷朝鲜蓟块茎组织似乎是一个典型的（细胞色素P-450）连接的单加氧酶。
A lauric acid monooxygenase which catalyzes the formation of hydroxylaurate from lauric acid has been characterized in ageing tissues of Jerusalem artichoke (Helianthus tuberosus L.) tuber. Three reaction products have been identified from the mass fragmentation pattern of their methyltrimethylsilyl derivatives: 10-hydroxylauric acid, 9-hydroxylauric acid and 8-hydroxylauric acid. Enzyme activity is located on the microsomal fraction which also carries cytochrome P-450 and NADPH cytochrome-c reductase. The apparent Km of the enzyme for lauric acid is 0.97 micronM. Laurate monooxygenation is dependent upon O2 and inhibited by CO. The latter effect is light reversible. NADPH is the preferred electron donor although appreciable NADH-sustained activity was observed. NADPH cytochrome c reductase is involved in electron transfer as evidenced by the inhibitory effects of NADP+ and oxidized cytochrome c on laurate monooxygenation. Thus, the enzyme catalyzing laurate oxidation in Jerusalem artichoke tuber tissues appears to be a typical (cytochrome P-450)-linked monooxygenase.