Identification of mouse Ly6H and its expression in normal tissue
Identification of mouse Ly6H and its expression in normal tissue
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DOI：
10.1007/s002510050583
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发表时间：
1999-10-01
期刊：
影响因子：
3.2
通讯作者：
Sandrin, MS
中科院分区：
文献类型：
--
作者：
Apostolopoulos, J;Chisholm, LJ;Sandrin, MS
Ly 6基因编码糖基磷脂酰肌醇锚定的细胞表面糖蛋白，其由髓样和淋巴样细胞表达，并且可能在T细胞活化中起作用。筛选小鼠表达序列标签（EST）数据库中与人Ly 6 H基因同源的序列。共鉴定出58条EST，其中10条含有长度大于20 bp的片段。这些序列用于产生小鼠Ly 6 H的预测cDNA序列。根据预测的序列设计引物，利用PCR技术从小鼠脑cDNA文库中分离到Ly 6 HcDNA。该基因编码160个氨基酸的蛋白质，包括3个翻译起始位点和一个N-连接糖基化位点。该基因与人类Ly 6 H基因的同源性为90%，相似性为96%。用北方印迹分析小鼠基因的表达;该基因在脑中强表达，在眼和生殖器官中弱表达。所报道的核苷酸序列已保藏于GenBank数据库，保藏号为AF 127091。
TheLy6genes encode glycosylphosphatidylinositol-anchored cell surface glycoproteins that are expressed by myeloid and lymphoid cells and may have a role in T-cell activation. A mouse expressed sequence tag (EST) database was screened for sequences homologous to the humanLy6Hgene. 58 ESTs were identified, 10 of which contained fragments >20 bp long. These sequences were used to generate a predicted cDNA sequence for mouseLy6H. PCR with primers based on this predicted sequence was used to isolate theLy6HcDNA from a mouse brain cDNA library. The gene encoded a protein of 160 amino acids and included 3 translation initiation sites and an N-linked glycosylation site. The gene had 90% identity and 96% similarity to humanLy6H. Northern blotting was used to analyse expression of the mouse gene; the gene was expressed strongly in brain and weakly in eye and reproductive organs. The nucleotide sequence reported has been deposited with the GenBank database with the accession number AF127091.