DNA methyltransferase-3 like protein expression in various histological types of testicular germ cell tumor.
DNA methyltransferase-3 like protein expression in various histological types of testicular germ cell tumor.
复制标题
DNA甲基转移酶3样蛋白在各种组织学类型的睾丸生殖细胞肿瘤中的表达。
DOI：
10.1093/jjco/hyw012
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发表时间：
2016
影响因子：
2.4
通讯作者：
Nishiyama H.
中科院分区：
文献类型：
--
作者：
Matsuoka T;Kawai K;Ando S;Sugita S;Kandori S;Kojima T;Miyazaki J;Nishiyama H.
cDNA甲基转移酶3-like在生殖细胞发育中起重要作用。本研究的目的是分析DNA甲基转移酶3样蛋白表达在睾丸生殖细胞tumors.MethodsThe免疫组化染色的DNA甲基转移酶3样蛋白表达在86睾丸生殖细胞肿瘤标本在不同的临床设置。DNA甲基转移酶3样表达和疾病分期之间的关联进行了analysed.ResultsDNA甲基转移酶3样强表达的8个纯胚胎癌（87.5%）。23例单纯性乳腺癌中6例（26.1%）有部分DNA甲基转移酶3样表达。不同程度的DNA甲基转移酶3样表达观察到在所有四个纯卵黄囊肿瘤，其中三个是青春期前卵黄囊肿瘤。在混合性生殖细胞肿瘤中，胚胎癌（14/18，77.8%）、卵巢上皮瘤（4/11，36.4%）、畸胎瘤（4/7，57.1%）和绒毛膜癌（3/3，100%）中均有不同程度的DNA甲基转移酶3样蛋白表达，而卵黄囊瘤中无表达（0/4）。当根据疾病分期分析DNA甲基转移酶3样表达时，它与晚期乳腺癌而不是I期乳腺癌显著相关，（46.2 vs.0%，P = 0.019），而在混合性生殖细胞肿瘤I期和晚期之间，DNA甲基转移酶3样阳性比例无显著差异。Like蛋白不仅在胚胎癌的发生中起作用，而且在晚期单纯性卵巢癌和单纯性卵黄囊瘤的发生中也起作用。
ObjectiveDNA methyltransferase 3-like plays an important role in germ cell development. The aim of this study was to analyse the DNA methyltransferase 3-like protein expression in testicular germ cell tumors.MethodsThe immunohistochemical expression of DNA methyltransferase 3-like was examined in 86 testicular germ cell tumor specimens in various clinical settings. The association between DNA methyltransferase 3-like expression and disease stage was analyzed.ResultsDNA methyltransferase 3-like was strongly expressed in seven of the eight pure embryonal carcinomas (87.5%). Partial DNA methyltransferase 3-like expression was observed in 6 of 23 (26.1%) pure seminomas. Various degrees of DNA methyltransferase 3-like expression was observed in all four pure yolk sac tumors, of which three were prepubertal yolk sac tumors. In mixed germ cell tumors, DNA methyltransferase 3-like protein was expressed in various degrees in elements of the embryonal carcinoma (14/18, 77.8%), seminoma (4/11, 36.4%), teratoma (4/7, 57.1%) and choriocarcinoma (3/3, 100%) but not in the yolk sac tumors (0/4). When DNA methyltransferase 3-like expression was analyzed according to disease stages, it was significantly correlated with advanced seminoma rather than Stage I seminoma (46.2 vs. 0%,P= 0.019), whereas there was no significant difference in the DNA methyltransferase 3-like-positive proportion between Stage I and advanced disease in the mixed germ cell tumors.ConclusionsOur findings suggest that DNA methyltransferase 3-like protein may play roles not only in the development of embryonal carcinoma but also in the development of advanced pure seminoma and pure yolk sac tumor.