AN ARABIDOPSIS HEAT-SHOCK PROTEIN COMPLEMENTS A THERMOTOLERANCE DEFECT IN YEAST
AN ARABIDOPSIS HEAT-SHOCK PROTEIN COMPLEMENTS A THERMOTOLERANCE DEFECT IN YEAST
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DOI：
10.1105/tpc.6.12.1899
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发表时间：
1994-12-01
期刊：
影响因子：
11.6
通讯作者：
VIERLING, E
中科院分区：
文献类型：
--
作者：
SCHIRMER, EC;LINDQUIST, S;VIERLING, E
酿酒酵母的热休克蛋白 Hsp104 在促进极端温度下的存活方面发挥着关键作用。我们发现，当多种高等植物物种暴露于高温时，它们会积累与 Hsp104 抗原相关的蛋白质。我们从拟南芥中分离出了与其中一种蛋白质相对应的 cDNA。 AtHSP101 蛋白与酵母 Hsp104 具有 43% 的一致性。 DNA 凝胶印迹分析表明 AtHSP101 由单拷贝数或低拷贝数基因编码。 AtHsp101 mRNA 在没有压力的情况下无法检测到，但在暴露于高温时会积累到很高的水平。当AtHSP101在酵母中表达时，它补充了HSP104基因缺失引起的耐热性缺陷。 AtHSP101 保护酵母免受严重热应激的能力强烈表明该 HSP 在高等植物的耐热性中发挥着重要作用。
The heat shock protein Hsp104 of the yeast Saccharomyces cerevisiae plays a key role in promoting survival at extreme temperatures. We found that when diverse higher plant species are exposed to high temperatures they accumulate proteins that are antigenically related to Hsp104. We isolated a cDNA corresponding to one of these proteins from Arabidopsis. The protein, AtHSP101, is 43% identical to yeast Hsp104. DNA gel blot analysis indicated that AtHSP101 is encoded by a single- or low-copy number gene. AtHsp101 mRNA was undetectable in the absence of stress but accumulated to high revels during exposure to high temperatures. When AtHSP101 was expressed in yeast, it complemented the thermotolerance defect caused by a deletion of the HSP104 gene. The ability of AtHSP101 to protect yeast from severe heat stress strongly suggests that this HSP plays an important role in thermotolerance in higher plants.