Phenotypic diversity among ruminal isolates of Prevotella ruminicola: Proposal of Prevotella brevis sp nov, Prevotella bryantii sp nov, and Prevotella albensis sp nov and redefinition of Prevotella ruminicola
Phenotypic diversity among ruminal isolates of Prevotella ruminicola: Proposal of Prevotella brevis sp nov, Prevotella bryantii sp nov, and Prevotella albensis sp nov and redefinition of Prevotella ruminicola
复制标题
DOI：
10.1099/00207713-47-2-284
复制
发表时间：
1997-04-01
期刊：
影响因子：
--
通讯作者：
Flint, HJ
中科院分区：
文献类型：
--
作者：
Avgustin, G;Wallace, RJ;Flint, HJ
为了确定先前基于16 S核糖体DNA序列建立的基因型菌株群的特征是否系统地不同，研究了瘤胃普雷沃氏菌（以前称为瘤胃拟杆菌）分离株的选定表型特征。在以前认为的瘤胃普氏菌短亚种的菌株中，与菌株GA 33（T）（T =型菌株）相关的菌株通常不能产生通过平板测定可检测的羧甲基纤维素酶（CMCase）活性，并且不能发酵木糖，而与菌株B（1）4（T）相关的菌株产生丰富的CMCase和发酵木糖。我们建议，与GA 33（T）相关的菌株，其DNA G + C含量在45 ~ 51 mol%之间，应归属于一个新种--短普雷沃氏菌（Prevotella brevis），而与B（1）4（T）相关的菌株，其DNA G + C含量在39 ~ 43 mol%之间，应归属于另一个新种。普氏菌大多数以前被归类为P. ruminicola亚种的分离物，ruminicola菌株产生CMCase，DNA G + C含量在45和51摩尔%之间，我们建议这些生物体应被置于重新定义的物种P. ruminicola中。一小部分G + C含量较低的菌株归属于另一个新种--白普雷沃氏菌（Prevotella albensis）。大多数短假单胞菌和贝氏假单胞菌菌株产生丰富的胞外DNA酶活性。蛋白酶活性（通过[C-14]酪蛋白水解测定）在菌株之间变化很大，短假单胞菌菌株表现出最高的平均活性。所有菌株产生二肽基肽酶活性，但对不同的肽底物的相对活性所表现出的P. bryantii，P. albensis，和P. brevis系统地不同。新定义的物种之间的表型差异表明，他们可能占据不同的生态位内瘤胃生态系统。
Selected phenotypic characteristics of isolates of Prevotella ruminicola (formerly Bacteroides ruminicola) were studied in order to establish whether the characteristics of genotypic strain groups established previously on the basis of 16S ribosomal DNA sequences differed systematically. Among strains formerly considered P. ruminicola subsp, brevis, strains related to strain GA33(T) (T = type strain) typically failed to produce carboxymethyl cellulase (CMCase) activity detectable by plate assays and failed to ferment xylose, while strains related to strain B(1)4(T) produced abundant CMCase and fermented xylose. We propose that strains related to GA33(T), which have DNA G + C contents between 45 and 51 mol%, should be assigned to a new species, Prevotella brevis, and that strains related to B(1)4(T), which have DNA G + C contents between 39 and 43 mol%, should be assigned to another new species. Prevotella bryantii. Most of the isolates formerly classified as P. ruminicola subsp, ruminicola strains produced CMCase and had DNA G + C contents between 45 and 51 mol%, and we propose that these organisms should be placed in the redefined species P. ruminicola. A small group of isolates that have lower G + C contents are assigned to another new species, Prevotella albensis. Most P. brevis and P. byrantii strains produced abundant extracellular DNase activity. Proteinase activities (as determined by [C-14]casein hydrolysis) varied widely between strains, and P. brevis strains exhibited the highest mean activity. All strains produced dipeptidyl peptidase activity, but the relative activities against different peptide substrates exhibited by P. bryantii, P. albensis, and P. brevis differed systematically. The phenotypic differences among the newly defined species suggest that they may occupy distinct niches within the rumen ecosystem.