Differential RIP antigen (CNPase) expression in peripheral ensheathing glia
Differential RIP antigen (CNPase) expression in peripheral ensheathing glia
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DOI：
10.1016/j.brainres.2006.12.053
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发表时间：
2007-03-16
期刊：
影响因子：
2.9
通讯作者：
Ramer, Matt S.
中科院分区：
文献类型：
--
作者：
Toma, Jeremy S.;McPhail, Lowell T.;Ramer, Matt S.
RIP单克隆抗体通常用于鉴定少突胶质细胞。最近，RIP抗原被鉴定为2 ′，3 ′-环核苷酸T-磷酸二酯酶（CND 2），一种已知的非紧密髓鞘蛋白[Watanabe，M.，Sakurai，Y.，Ichinose，T.，Aikawa，Y.，Kotani，M.，Itoh，K.，2006.单克隆抗体Rip特异性识别少突胶质细胞中的2 '，3'-环核苷酸3 '-磷酸二酯酶。神经科学杂志Res. 84，525-533]。在本研究中，我们的特点正常和轴突切断诱导的变化RIP免疫反应性在周围神经胶质细胞。在有髓鞘的雪旺氏细胞中，RIP划定了有髓鞘轴突的结旁区域和明确定义的施密特-兰特曼切口。令人惊讶的是，RIP免疫反应并不局限于髓鞘神经胶质。稳健RIP免疫反应存在于混合神经的Remak束和交感神经节和灰支。周围神经损伤后，RIP免疫反应性在整个去分化雪旺细胞胞质中弥漫性分布。在未受伤的大鼠，低水平的RIP免疫反应性可检测到卫星细胞周围的背根神经节（DRG）神经元和神经肌肉接头的终端许旺细胞。这种模式表明RIP免疫反应性和轴突-神经胶质细胞接触量之间的相关性。因此，我们伤害了L5脊神经，诱导交感神经发芽和细胞周围的篮子形成在DRG中，并询问是否相对RIP阴性的卫星胶质细胞，通常只接触神经元胞体，将上调RIP抗原与发芽的交感神经轴突接触后。所有神经元周围的交感神经芽浸润严重RIP免疫反应卫星细胞鞘。基板来源的嗅鞘神经胶质中不存在RIP免疫反应性，这表明轴突-神经胶质接触和RIP免疫反应性之间的关系仅限于神经嵴来源的施万细胞谱系的外周鞘神经胶质。(c)2006 Elsevier B. V.保留所有权利。
The RIP monoclonal antibody is commonly used to identify oligodendrocytes. Recently, the RIP antigen was identified as 2',3'-cyclic nucleotide T-phosphodiesterase (CNPase), a known non-compact myelin protein [Watanabe, M., Sakurai, Y., Ichinose, T., Aikawa, Y., Kotani, M., Itoh, K., 2006. Monoclonal antibody Rip specifically recognizes 2',3'-cyclic nucleotide 3'-phosphodiesterase in oligodendrocytes. J. Neurosci. Res. 84, 525-533]. In the present study we characterize normal and axotomy-induced changes in RIP immunoreactivity in peripheral glia. In myelinating Schwann cells, RIP demarcated paranodal regions of myelinated axons and clearly defined Schmidt-Lantermann incisures. Surprisingly, RIP immunoreactivity was not confined to myelinating glia. Robust RIP immunoreactivity was present in Remak bundles in mixed nerves and in sympathetic ganglia and grey rami. Following peripheral nerve injury, RIP immunoreactivity was redistributed diffusely throughout de-differentiating Schwann cell cytoplasm. In uninjured rats, low levels of RIP immunoreactivity were detectable in satellite cells surrounding dorsal root ganglion (DRG) neurons and in terminal Schwann cells at neuromuscular junctions. This pattern suggested a correlation between RIP immunoreactivity and the amount of axon-glial contact. We therefore injured the L5 spinal nerve to induce sympathetic sprouting and pericellular basket formation in the DRG, and asked whether relatively RIP-negative satellite glia, which normally contact only neuronal somata, would upregulate the RIP antigen upon contact with sprouting sympathetic axons. All perineuronal sympathetic sprouts infiltrated heavily RIP-immunoreactive satellite cell sheaths. RIP immunoreactivity was absent from placode-derived olfactory ensheathing glia, indicating that the relationship between axon-glial contact and RIP-immunoreactivity is restricted to peripheral ensheathing glia of the neural crest-derived Schwann cell lineage. (c) 2006 Elsevier B.V. All rights reserved.