Reinitiation of synthesis of small cytoplasmic RNA species K and L in isolated HeLa cell nuclei in vitro.
Reinitiation of synthesis of small cytoplasmic RNA species K and L in isolated HeLa cell nuclei in vitro.
复制标题
在体外分离的 HeLa 细胞核中重新启动小细胞质 RNA K 和 L 的合成。
作者：
R. Reichel;B. Benecke
分离的HeLa细胞核用于体外合成低分子量RNA种类。孵育过程中从细胞核释放的标记RNA主要由5S RNA、前tRNA和小的胞质RNA种类K和L组成。所有这些低分子量RNA种类都由RNA聚合酶C（或III）合成。应用聚阴离子肝素研究这些RNA分子的体外再起始。在不存在和存在该抑制剂的情况下RNA合成动力学的比较证明了通过RNA聚合酶C对scRNA种类K和L以及5S和pre-tRNA的高效体外再起始。这些结果表明该酶在体外催化特定基因产物从头形成的一般能力。
Isolated HeLa cell nuclei were used to synthesize low molecular weight RNA species in-vitro. The labelled RNA released from the nuclei during the incubation mainly consists of 5S RNA, pre-tRNA and small cytoplasmic RNA species K and L. All these low molecular weight RNA species are synthesized by RNA polymerase C (or III). The polyanion heparin was applied to study the reinitiation of these RNA molecules in-vitro. A comparison of the kinetics of RNA synthesis in the absence and in the presence of this inhibitor demonstrates a highly efficient in-vitro reinitiation of scRNA species K and L as well as 5S and pre-tRNA by RNA polymerase C. These results indicate a general competence of this enzyme to catalyze the de-novo formation of specific gene products in-vitro.