An asymmetric field inversion gel electrophoresis method for the separation of large DNA molecules.
An asymmetric field inversion gel electrophoresis method for the separation of large DNA molecules.
复制标题
一种用于分离大 DNA 分子的不对称场反转凝胶电泳方法。
DOI：
10.1016/0003-2697(89)90375-8
复制
发表时间：
1989
影响因子：
2.9
通讯作者：
Stamato,TD
中科院分区：
文献类型：
--
作者：
Denko,N;Giaccia,A;Peters,B;Stamato,TD
描述了一种用于分离大DNA分子的电泳方法，该方法在两个方向上使用不同大小的周期性倒置电场。净DNA迁移是在高场方向或低场方向，这取决于脉冲的相对持续时间。通过这种方法，在固定的时间条件下，单次运行后，多达160万个碱基对的分子可以在平行通道中分离。除了传统的凝胶盒之外，唯一需要的设备是一个便宜的开关单元。
An electrophoretic method for separating large DNA molecules which uses periodically inverted electric fields of different magnitude in the two directions is described. Net DNA migration is either in the high field direction or in the low field direction, depending on the relative duration of the pulses. With this approach, molecules of up to 1.6 million base pairs can be separated in parallel lanes after a single run under fixed timing conditions. An inexpensive switching unit is the only device needed in addition to the conventional gel box.