Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation
Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation
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DOI：
10.1073/pnas.1101503108
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发表时间：
2011-03-22
影响因子：
11.1
通讯作者：
Frank, Joachim
中科院分区：
文献类型：
--
作者：
Fu, Jie;Munro, James B.;Frank, Joachim
mRNA-tRNA易位是翻译延伸过程中一个高度调控的中心过程。与mRNA一起，tRNA以一种循序渐进的方式穿过核糖体。利用低温电子显微镜观察具有p环突变的核糖体，我们发现了可能在易位过程中短暂存在的新型结构中间体。我们的观察结果提示了一种可以调节易位速率的机制。
mRNA-tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion. Using cryoelectron microscopy on ribosomes with a P-loop mutation, we have identified novel structural intermediates likely to exist transiently during translocation. Our observations suggest a mechanism by which the rate of translocation can be regulated.