Trichoderma virens, a Plant Beneficial Fungus, Enhances Biomass Production and Promotes Lateral Root Growth through an Auxin-Dependent Mechanism in Arabidopsis
Trichoderma virens, a Plant Beneficial Fungus, Enhances Biomass Production and Promotes Lateral Root Growth through an Auxin-Dependent Mechanism in Arabidopsis
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DOI：
10.1104/pp.108.130369
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发表时间：
2009-03-01
期刊：
影响因子：
7.4
通讯作者：
Lopez-Bucio, Jose
中科院分区：
文献类型：
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作者：
Angel Contreras-Cornejo, Hexon;Macias-Rodriguez, Lourdes;Lopez-Bucio, Jose
木霉属物种属于一类对植物有益的自由生活真菌，其在根际中常见。通过建立植物-真菌互作系统，研究了生长素在绿木霉和深绿木霉诱导下对拟南芥幼苗生长发育的调控作用。野生型拟南芥幼苗接种T. virens或T.深绿素表现出特征性的生长素相关表型，包括增加生物量生产和刺激侧根发育。参与生长素运输或信号传导的基因AUX 1、BIG、EIR 1和AXR 1的突变被发现降低了T.病毒接种。在无菌条件下生长，T.绿色植物产生生长素相关化合物吲哚-3-乙酸、吲哚-3-乙醛和吲哚-3-乙醇。所有三个吲哚类化合物的比较分析提供了详细的信息的结构-活性关系的基础上，它们的功效在调节根系结构，激活生长素调节的基因表达，和救援的rhd 6生长素反应拟南芥突变体的根毛缺陷表型。我们的研究结果强调了生长素信号在T.绿色的
Trichoderma species belong to a class of free-living fungi beneficial to plants that are common in the rhizosphere. We investigated the role of auxin in regulating the growth and development of Arabidopsis ( Arabidopsis thaliana) seedlings in response to inoculation with Trichoderma virens and Trichoderma atroviride by developing a plant-fungus interaction system. Wild-type Arabidopsis seedlings inoculated with either T. virens or T. atroviride showed characteristic auxin-related phenotypes, including increased biomass production and stimulated lateral root development. Mutations in genes involved in auxin transport or signaling, AUX1, BIG, EIR1, and AXR1, were found to reduce the growth-promoting and root developmental effects of T. virens inoculation. When grown under axenic conditions, T. virens produced the auxin-related compounds indole-3-acetic acid, indole-3-acetaldehyde, and indole-3-ethanol. A comparative analysis of all three indolic compounds provided detailed information about the structure-activity relationship based on their efficacy at modulating root system architecture, activation of auxin-regulated gene expression, and rescue of the root hair-defective phenotype of the rhd6 auxin response Arabidopsis mutant. Our results highlight the important role of auxin signaling for plant growth promotion by T. virens.