Methodologic recommendations and possible interpretations of video-EEG recordings in immaturerodents used as experimental controls: ATASK1-WG2 report of the ILAE/AES Joint TranslationalTask Force.
Methodologic recommendations and possible interpretations of video-EEG recordings in immaturerodents used as experimental controls: ATASK1-WG2 report of the ILAE/AES Joint TranslationalTask Force.
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DOI：
10.1002/epi4.12262
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发表时间：
2018-12-01
期刊：
影响因子：
3
通讯作者：
Galanopoulou, Aristea S
中科院分区：
文献类型：
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作者：
Akman, Ozlem;Raol, Yogendra H;Galanopoulou, Aristea S
使用未成熟的啮齿动物研究皮层发育的生理方面需要高质量的脑电图（EEG）记录和行为同步视频记录（vEEG）。对照动物的正常发育 vEEG 数据对于研究癫痫或其他神经系统疾病动物模型中的异常背景活动至关重要。由于未成熟啮齿动物体型小、头骨薄而软、水坝对记录装置的干扰以及其他技术挑战，对未成熟、自由行为的啮齿动物进行电记录可能特别困难。在国际抗癫痫联盟/美国癫痫协会 (ILAE/AES) 联合转化工作组 TASK1 工作组 2 (WG2) 的这份报告中，我们提供了旨在优化未成熟啮齿动物未来 vEEG 记录及其解释的建议。我们重点关注用作实验对照的 30 天以下的未成熟啮齿动物的记录，因为在解释作为神经系统疾病模型的动物的 vEEG 结果时，此类记录的质量和正确解释非常重要。我们讨论此类录音的技术方面，并比较有线方法与无线方法。我们还总结了在年轻啮齿动物中看到的常见伪影的外观和各种电活动模式，这些啮齿动物用作对照，作为行为状态、年龄和（已知的）性别和应变的函数。本文的信息有望有助于改进未成熟啮齿动物的 vEEG 记录方法，并可能导致不同实验室的研究结果和解释更加一致。
The use of immature rodents to study physiologic aspects of cortical development requires high-quality recordings electroencephalography (EEG) with simultaneous video recording (vEEG) of behavior. Normative developmental vEEG data in control animals are fundamental for the study of abnormal background activity in animal models of seizures or other neurologic disorders. Electrical recordings from immature, freely behaving rodents can be particularly difficult because of the small size of immature rodents, their thin and soft skull, interference with the recording apparatus by the dam, and other technical challenges. In this report of the TASK1 Working Group 2 (WG2) of the International League Against Epilepsy/American Epilepsy Society (ILAE/AES) Joint Translational Task Force, we provide suggestions that aim to optimize future vEEG recordings from immature rodents, as well as their interpretation. We focus on recordings from immature rodents younger than 30days old used as experimental controls, because the quality and correct interpretation of such recordings is important when interpreting the vEEG results of animals serving as models of neurologic disorders. We discuss the technical aspects of such recordings and compare tethered versus wireless approaches. We also summarize the appearance of common artifacts and various patterns of electrical activity seen in young rodents used as controls as a function of behavioral state, age, and (where known) sex and strain. The information herein will hopefully help improve the methodology of vEEG recordings from immature rodents and may lead to results and interpretations that are more consistent across studies from different laboratories.