Donor platelets stored for at least 3 days can elicit activation marker expression by the recipient's blood mononuclear cells: an in vitro study
Donor platelets stored for at least 3 days can elicit activation marker expression by the recipient's blood mononuclear cells: an in vitro study
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DOI：
10.1111/j.1537-2995.2008.01931.x
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发表时间：
2009-01-01
期刊：
影响因子：
2.9
通讯作者：
Garraud, Olivier
中科院分区：
文献类型：
--
作者：
Cognasse, Fabrice;Hamzeh-Cognasse, Hind;Garraud, Olivier
背景：最近的研究表明，输注血小板（PLTs）可以促进炎症。本研究的目的是评估输注级plt的储存对受体外周血单核细胞（pbmnc）的影响。研究设计与方法：建立体外细胞模型系统，测定供体plt储存5 d后的活化程度，并在共培养实验中通过检测标志物表达来测定免疫细胞的活化程度。结果：可溶性CD62p水平在第3天显著升高，CD62p的膜表达从第2天开始显著升高，表明随着储存时间的推移，PLT有一定程度的活化（p < 0.05）。来自受体的供体plt和PBMNC亚群（单核细胞、B细胞和T细胞）共培养48小时。流式细胞术检测到的PLT- pbmnc亚群双偶数目与第3天后PLT储存时间相关（p < 0.05），表明PLT与pbmnc结合一致。这些实验结果表明，与适当的对照组相比，T细胞、B细胞和单核细胞的常规活化标志物的表达一致且显著增加（p < 0.05 ~ < 0.01）。结论：本研究结果表明，从第3天开始，激活标记物在plt上一致表达。根据这些结果，我们得出结论，活化的plt可能影响受体中PBMNC的相互作用。
BACKGROUND: Recent studies have demonstrated that infused platelets (PLTs) can promote inflammation. The objective of this study was to evaluate the impact of storage of transfusion-grade PLTs on the peripheral blood mononuclear cells (PBMNCs) of the recipient.STUDY DESIGN AND METHODS: An in vitro cell model system was established to measure the degree of activation of donor PLTs during 5 days of their storage and then to measure immune cell activation by detecting marker expression in coculture experiments.RESULTS: The level of soluble CD62p increased significantly by Day 3, and membrane expression of CD62p increased significantly from Day 2, indicating some degree of PLT activation over time during storage (p < 0.05). Donor PLTs and PBMNC subsets (monocytes, B cells, and T cells) from recipients were cocultured for 48 hours. The number of PLT-PBMNC subset doublets detected by flow cytometry was correlated with the PLT storage time after Day 3 (p < 0.05), indicating consistent binding of PLTs to PBMNCs. The results of these experiments showed that there was a consistent and significant increase in expression of conventional activation markers of T cells, B cells, and monocytes compared with appropriate controls (p < 0.05 to < 0.01).CONCLUSION: The results of this study indicate that, from Day 3 onward, activation markers are consistently expressed on PLTs. From these results, we conclude that activated PLTs may affect PBMNC interactions in recipients.