Separation and purification of hydroxytysol and oleuropein from Olea europaea L. (olive) leaves using macroporous resins and a novel solvent system
Separation and purification of hydroxytysol and oleuropein from Olea europaea L. (olive) leaves using macroporous resins and a novel solvent system
复制标题
DOI：
10.1002/jssc.201901227
复制
发表时间：
2020-05-25
影响因子：
3.1
通讯作者：
Di, Duolong
中科院分区：
文献类型：
--
作者：
Liu, Baoqian;Liu, Jianfei;Di, Duolong
采用大孔树脂和新型溶剂体系对橄榄中羟基醇和橄榄苦苷进行了分离纯化。在BMKX-4树脂上进行静态吸附实验，结果表明，羟基tysol和油梨苦苷均符合拟二级动力学模型和Freundlich等温线模型。热力学参数表现为自发吸附和放热吸附。该新型溶剂体系由正己烷：乙酸乙酯：甲醇：水组成，（v/v/v/v）比为1:9:1:9，有上、下两相。在BMKX-4树脂填充柱上，采用动态吸附/解吸技术，优化了羟基tysol和橄榄苦苷的分离纯化参数。系统地研究了上、下相流速和体积对分离效率的影响。在最优条件下，最终产物中羟tysol的含量从0.46提高到2.96%，收率为88.58%；橄榄苦苷的含量从11.40提高到47.59%，收率为93.31%。这些结果可能有助于选择合适的洗脱液来改善大孔吸附树脂的分离。
The separation and purification of hydroxytysol and oleuropein from Olea europaea L. (olive) using a macroporous resin with a novel solvent system was systematically investigated. Static adsorption experiments with BMKX-4 resin revealed that the experimental data of both hydroxytysol and oleuropein fitted best to the pseudo-second-order kinetic and Freundlich isotherm models. The thermodynamic parameters indicated spontaneous and exothermic adsorption processes. The novel solvent system, composed of n-hexane:ethyl acetate:methanol:water in a (v/v/v/v) ratio of 1:9:1:9, had two phases (upper and lower). The separation and purification parameters of hydroxytysol and oleuropein were optimized using dynamic adsorption/desorption on a column packed with BMKX-4 resin. The effects of flow rates and volumes of the upper and lower phases on the separation efficiency were systematically studied. Under optimal conditions, the fraction of hydroxytysol in the final product increased by 6.34-fold from 0.46 to 2.96%, with a yield rate of 88.58% w/w, while that of oleuropein increased 4.17-fold from 11.40 to 47.59%, with a 93.31% w/w yield rate. These results may be help in selecting a suitable eluent for improved separation of macroporous adsorption resins.