EFFECT OF MITOTIC INHIBITORS ON MYOGENESIS IN VITRO
EFFECT OF MITOTIC INHIBITORS ON MYOGENESIS IN VITRO
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DOI：
10.1083/jcb.36.1.111
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发表时间：
1968-01-01
影响因子：
7.8
通讯作者：
HOLTZER, H
中科院分区：
文献类型：
--
作者：
BISCHOFF, R;HOLTZER, H
研究了秋水仙碱和其他抗有丝分裂药物在11日龄鸡胚胸肌培养中的作用。将这种培养物暴露于10-6[图像]秋水仙碱中会导致细长的肌管碎裂成圆形的、含有不同数量细胞核的细胞质囊（肌囊）。对肌管断裂和中期停搏的剂量-反应关系的比较表明，这两种情况的潜在机制可能相似。低温不能复制秋水仙碱的效果。糖基化肌管不受有丝分裂抑制剂影响。秋水仙碱对肌管的作用是可逆的。肌囊在去除秋水仙碱后几天内拉长。然而，再生的肌管不能合并额外的单核细胞。秋水仙碱本身不干扰融合过程，但中期阻滞阻止细胞进入可以发生融合的细胞周期阶段。在有丝分裂中被秋水仙碱抑制的细胞在正常培养基中孵育时不能恢复。秋碱诱导的细胞阻滞是可逆的，不妨碍细胞的后续融合。
The effect of colchicine and other antimitotic drugs was studied in cultures of 11-day chick embryo breast muscle. Exposure of such cultures to 10-6 [image] colchicine results in fragmentation of the elongate myotubes into rounded, cytoplasmic sacs (myosacs) containing various numbers of nuclei. Comparison of the dose-response relation between myotube fragmentation and metaphase arrest suggests that the underlying mechanism may be similar in both cases. Low temperature does not duplicate the effects of colchicine. Gly-cerinated myotubes are not affected by the mitotic inhibitors. The effect of colchicine on myotubes is reversible. Myosacs elongate within several days after removal from colchicine. However, the regenerated myotubes fail to incorporate additional mononucleated cells. Colchicine does not interfere with the process of fusion itself, but the metaphase block prevents cells from entering that phase of the cell cycle during which fusion can occur. Cells arrested in mitosis by colchicine do not recover when incubated in normal medium. Colcemid-induced arrest is reversible and does not prevent subsequent fusion of the cells.