Protein-G-based human immunoglobulin G biosensing by electrochemical impedance spectroscopy
Protein-G-based human immunoglobulin G biosensing by electrochemical impedance spectroscopy
复制标题
DOI：
10.7567/jjap.55.02be06
复制
发表时间：
2016-01
影响因子：
1.5
通讯作者：
Kaiki Tsugimura;H. Ohnuki;H. Endo;Daijyu Tsuya;M. Izumi
中科院分区：
文献类型：
--
作者：
Kaiki Tsugimura;H. Ohnuki;H. Endo;Daijyu Tsuya;M. Izumi
研制了一种基于电化学阻抗谱（EIS）的高灵敏度生物传感器，用于人免疫球蛋白G（IgG）的测定。采用特异性结合IgG的蛋白G作为分子受体。蛋白G通过由11-巯基十一烷酸（MUA）和6-巯基己醇组成的混合自组装单分子膜（SAM）共价固定在叉指电极上。结果发现，SAM的混合比例显着影响传感器的性能。在25% MUA SAM上制备的样品在0.01-10 ng/mL的浓度范围内表现出线性行为，这是基于EIS的IgG传感器的创纪录低检测。另一方面，100%MUA SAM上的样品未显示IgG敏感作用。提出了混合比影响传感性能的可能机理。
A highly sensitive biosensor based on electrochemical impedance spectroscopy (EIS) was developed for the determination of human immunoglobulin G (IgG). Protein G, which specifically binds to IgG, was employed as the molecular receptor. Protein G was covalently immobilized on interdigitated electrodes through a mixed self-assembled monolayer (SAM) composed of 11-mercaptoundecanoic acid (MUA) and 6-mercaptohexanol. It was found that the mixing ratio of the SAM markedly affected the sensor performance. The sample prepared on 25% MUA SAM exhibited a linear behavior in the concentration range of 0.01–10 ng/mL, which is a record low detection for EIS-based IgG sensors. On the other hand, the sample on 100% MUA SAM showed no IgG-sensing action. A possible mechanism of the mixing ratio that affects the sensing performance was proposed.