Uptake and selective partitioning of dietary lipids to ovarian and muscle tissue of maturing female coho salmon, Oncorhynchus kisutch, during secondary oocyte growth
Uptake and selective partitioning of dietary lipids to ovarian and muscle tissue of maturing female coho salmon, Oncorhynchus kisutch, during secondary oocyte growth
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DOI：
10.1016/j.cbpb.2017.03.005
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发表时间：
2017-06-01
影响因子：
2.2
通讯作者：
Rust, Michael B.
中科院分区：
文献类型：
--
作者：
Johnson, Ronald B.;Kroeger, Eric L.;Rust, Michael B.
雌性银鲑，Oncorhynchus kisutch，喂两个实验饲料含有脂质与显着不同的稳定的C-13同位素签名在后期皮质肺泡，脂滴，和卵黄发生阶段的次级卵母细胞生长。卵巢和肌肉脂质脂肪酸浓度显着影响治疗在所有三个发展阶段。稳定的C-13同位素分析证实，膳食脂质被纳入卵巢和肌肉脂质在所有三个阶段，并显示卵巢脂质比肌肉脂质在卵黄发生过程中受到更大的影响。花生四烯酸（ARA）被纳入卵巢脂质中的所有脂肪酸的最高速率检查与最大的吸收过程中观察到的皮质肺泡和脂滴阶段的发展。二十二碳六烯酸（DHA）被纳入卵巢脂质在第二个最高的速度与最大的吸收观察期间的脂滴阶段的发展。提出了卵巢特异性脂肪酸转移机制的存在。这项研究的结果表明，通过在次级卵母细胞生长过程中改变饮食，能够极大地改变卵巢脂质的脂肪酸组成，这可能会引起养殖鲑鱼和鲑鱼亲鱼生产者的极大兴趣。
Female coho salmon, Oncorhynchus kisutch, were fed one of two experimental feeds containing lipids with markedly different stable C-13 isotope signatures during the late cortical alveolus, lipid droplet, and vitellogenesis stages of secondary oocyte growth. Ovarian and muscle lipids fatty acid concentrations were significantly affected by treatment during all three stages of development. Stable C-13 isotope analyses confirmed that dietary lipids were incorporated into both ovarian and muscle lipids during all three stages and revealed that ovarian lipids were more affected than muscle lipids during vitellogenesis. Arachidonic acid (ARA) was incorporated into ovarian lipids at the highest rate of all fatty acids examined with the greatest uptake observed during the cortical alveolus and lipid droplet stages of development. Docosahexaenoic acid (DHA) was incorporated into ovarian lipids at the next highest rate with the greatest uptake observed during the lipid droplet stage of development. The presence of an ovary specific, fatty acid transfer mechanism is proposed. Results from this study demonstrate the ability to greatly alter the fatty acid composition of ovarian lipids through a dietary change during secondary oocyte growth and may be of great interest to producers of farmed salmon and salmon broodstock programs.