Reduction of Ribosome Level Triggers Flocculation of Fission Yeast Cells
Reduction of Ribosome Level Triggers Flocculation of Fission Yeast Cells
复制标题
核糖体水平的降低引发裂殖酵母细胞的絮凝
DOI：
10.1128/ec.00321-12
复制
发表时间：
2013-03-01
期刊：
影响因子：
--
通讯作者：
Yuan, Sheng
中科院分区：
文献类型：
--
作者：
Li, Rongpeng;Li, Xuesong;Yuan, Sheng
核糖体蛋白L32基因的缺失导致裂殖酵母的无性絮凝。当另外两个核糖体蛋白基因rpl 21 -2和rpl 9 -2缺失时，也会发生非性絮凝。然而，缺失两个非核糖体蛋白基因，mpg和fbp，没有引起絮凝。与野生型细胞相比，rpl 32 -1 Delta和rpl 32 -2 Delta细胞中rpl 32的总体转录物水平分别降低35.9%和46.9%，并且rpl 32 -1 Delta和rpl 32 -2 Delta细胞中的总体核糖体水平分别下降31.1%和27.8%。有趣的是，与YHL 6381（h（+））和WT（h（-））非二倍体细胞的混合物相比，在有性二倍体YHL 6381/WT（h（+）/h（-））细胞中核糖体蛋白表达水平和核糖体水平也大大降低。转录组分析表明，在有性生殖细胞中核糖体水平的降低是由于更广泛的抑制核糖体生物合成基因的表达，而在非有性生殖细胞中删除核糖体蛋白基因引起的核糖体水平的降低是由于核糖体蛋白之间的不平衡。我们建议，一旦核糖体水平的降低低于一定的阈值，絮凝被触发。
Deletion of ribosomal protein L32 genes resulted in a nonsexual flocculation of fission yeast. Nonsexual flocculation also occurred when two other ribosomal protein genes, rpl21-2 and rpl9-2, were deleted. However, deletion of two nonribosomal protein genes, mpg and fbp, did not cause flocculation. Overall transcript levels of rpl32 in rpl32-1 Delta and rpl32-2 Delta cells were reduced by 35.9% and 46.9%, respectively, and overall ribosome levels in rpl32-1 Delta and rpl32-2 Delta cells dropped 31.1% and 27.8%, respectively, compared to wild-type cells. Interestingly, ribosome protein expression levels and ribosome levels were also reduced greatly in sexually flocculating diploid YHL6381/WT (h(+)/h(-)) cells compared to a mixture of YHL6381 (h(+)) and WT (h(-)) non-flocculating haploid cells. Transcriptome analysis indicated that the reduction of ribosomal levels in sexual flocculating cells was caused by more-extensive suppression of ribosomal biosynthesis gene expression, while the reduction of ribosomal levels caused by deleting ribosomal protein genes in nonsexual flocculating cells was due to an imbalance between ribosomal proteins. We propose that once the reduction of ribosomal levels is below a certain threshold value, flocculation is triggered.