Construction of a genetic map for Caulobacter crescentus
Construction of a genetic map for Caulobacter crescentus
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新月柄杆菌遗传图谱的构建
DOI：
10.1128/jb.149.3.889-896.1982
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发表时间：
1982
影响因子：
3.2
通讯作者：
B. Ely
中科院分区：
文献类型：
--
作者：
J. T. Barrett;C. Rhodes;D. M. Ferber;B. Jenkins;S. A. Kuhl;B. Ely
RP 4介导的接合已被用于转移新月柄杆菌中的染色体材料的大片段。在该系统中，接合从多个来源进行，并且以每个供体细胞10（-6）和10（-7）的频率回收单倍体重组体。从五因素杂交的数据进行计算机辅助交叉分析作为一种快速的方法来确定标记顺序。利用这些信息和由RP 4或通用转导噬菌体phi Cr 30介导的额外的二因子和三因子杂交的数据，我们构建了第一个C. crescentus。
RP4-mediated conjugation has been used to transfer large fragments of chromosomal material in Caulobacter crescentus. In this system, conjugation proceeds from multiple origins, and haploid recombinants are recovered at frequencies of 10(-6) and 10(-7) per donor cell. The data from five-factor crosses were subjected to computer-assisted crossover analyses as a rapid method to determine marker order. Using this information and data from additional two- and three-factor crosses mediated by RP4 or the generalized transducing bacteriophage phi Cr30, we constructed the first genetic map for C. crescentus.