A label-free luminescent assay for tyrosinase activity monitoring and inhibitor screening with responsive lanthanide coordination polymer nanoparticles
A label-free luminescent assay for tyrosinase activity monitoring and inhibitor screening with responsive lanthanide coordination polymer nanoparticles
复制标题
利用响应性镧系元素配位聚合物纳米颗粒进行酪氨酸酶活性监测和抑制剂筛选的无标记发光测定
DOI：
10.1016/j.saa.2019.117751
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发表时间：
2020-03-05
影响因子：
4.4
通讯作者：
Wu, Fang-Ying
中科院分区：
文献类型：
--
作者：
Tian, Yao;Zhang, Zhipeng;Wu, Fang-Ying
本工作利用一种稀土配位聚合物纳米粒子AMP-Tb/Ag+，建立了一种无标记、高选择性、高灵敏度的酪氨酸酶（Tyrosinase，TYR）活性监测及其抑制剂筛选的荧光传感平台。利用AMP-Tb网络中Ag+和模型底物酪胺的产物多巴胺（DA）的特异性结合和氧化还原性质，将酶促反应和传感平台的信号变化有效地联系起来。通过改变Ag+的电子结构减弱AMP向Tb 3+的能量传递，以及多巴醌引起的有效的光诱导电子转移（PET）过程的协同作用，促进了Tb 3+的发光猝灭。因此，该发光传感平台可用于定量评价TYR活性。TYR的线性范围为0.08 ~ 0.20 U mL（-1），检出限为0.004 U mL（-1）。该方法已成功应用于人血清样品中TYR活性的准确测定和TYR抑制剂的高效筛选。由于镧系元素独特的光谱特性沿着操作简单和上级的分析性能，该传感平台在TYR相关疾病的临床诊断和药物筛选方面具有很大的应用前景。(c)2019爱思唯尔B. V.保留所有权利。
In this work, a label-free, selective, and sensitive luminescent sensing platform was established for tyrosinase (TYR) activity monitoring and its inhibitor screening using one kind of lanthanide coordination polymer nanoparticles AMP-Tb/Ag+. By taking advantage of the specific binding and redox properties of Ag+ incorporated into the AMP-Tb network and dopamine (DA) as the product of the model substrate tyramine, the enzymatic reaction and the signal change of the sensing platform was effectively linked. The cooperative effect of a weakened energy transfer from AMP to Tb3+ by altering the electronic structure of Ag+ and an efficient photoinduced election transfer (PET) process caused by dopaquinone facilitated the luminescence quenching of Tb3+. Thus, this luminescent sensing platform could be employed for quantitative evaluation of TYR activity. There was a good linear range for TYR activity from 0.08 to 0.20 U mL(-1) with a low detection limit of 0.004 U mL(-1). Furthermore, this assay was successfully applied to accurate determination of TYR activity in human serum samples and efficient screening of TYR inhibitors. Considering unique spectral characteristics of lanthanides along with operation simplicity and superior analytical performance, this sensing platform is very promising in clinical diagnosis and drugs screening for TYR-associated diseases. (c) 2019 Elsevier B.V. All rights reserved.