Fluorogenic Probes for Multicolor Imaging in Living Cells
Fluorogenic Probes for Multicolor Imaging in Living Cells
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DOI：
10.1021/jacs.6b04782
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发表时间：
2016-08-03
影响因子：
15
通讯作者：
Johnsson, Kai
中科院分区：
文献类型：
--
作者：
Lukinavicius, Grazvydas;Reymond, Luc;Johnsson, Kai
在这里，我们提出了一个远红外，硅罗丹明为基础的荧光团（SiR 700）的活细胞荧光成像。SiR 700分别在690和715 nm处具有激发和发射最大值。基于SiR 700的F-肌动蛋白、微管、溶酶体和SNAP标签探针是荧光的、细胞可渗透的，并且与超分辨率显微镜兼容。与基于之前引入的羧基SiR 650的探针结合，基于SiR 700的探针可以在远红外区进行多色活细胞超分辨率显微镜检查，从而显着扩展了我们对活细胞成像的能力。
Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-pertneable, and compatible with super resolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.