TUNING ELECTROSPINNING PARAMETERS FOR PRODUCTION OF 3D-FIBER-FLEECES WITH INCREASED POROSITY FOR SOFT TISSUE ENGINEERING APPLICATIONS
TUNING ELECTROSPINNING PARAMETERS FOR PRODUCTION OF 3D-FIBER-FLEECES WITH INCREASED POROSITY FOR SOFT TISSUE ENGINEERING APPLICATIONS
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DOI：
10.22203/ecm.v021a22
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发表时间：
2011-01-01
影响因子：
3.1
通讯作者：
Hall, Heike
中科院分区：
文献类型：
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作者：
Milleret, Vincent;Simona, Benjamin;Hall, Heike
Degrapol(R)和PLGA电纺纤维从纤维直径、排列、机械性能以及支架孔隙率等方面进行了表征。研究表明，电纺参数对纤维直径和取向的影响呈反比关系：纤维直径随流量的增加而增大，随工作距离和集束速度的减小而减小，纤维的取向随工作距离和集束速度的增加而增加，但随流量的增加而减小。当Degraol(R)或PLGA-聚合物随着水溶性聚合物比例的增加而共纺时，随后被移除；获得了孔隙率增加的纤维支架。力学性能与纤维取向相关，而不是与纤维直径相关，因为取向的纤维支架表现出很强的力学各向异性。对于共纺纤维，杨氏模数与共纺聚合物的量成反比。细胞增殖与支架的孔隙率无关，但两种聚合物的细胞增殖情况不同。此外，对具有不同孔隙率的纤维支架进行了细胞渗透分析，结果表明，细胞渗透随着孔隙率的增加和时间的延长而增强。这些实验表明，3D纤维羊毛可以在可控的性能下生产，这是在组织工程应用中成功构建支架的先决条件。
Degrapol (R) and PLGA electrospun fiber fleeces were characterized with regard to fiber diameter, alignment, mechanical properties as well as scaffold porosity. The study showed that electrospinning parameters affect fiber diameter and alignment in an inverse relation: fiber diameter was increased with increased flow rate, with decrease in working distance and collector velocity, whereas fiber alignment increased with the working distance and collector velocity but decreased with increased flow rate. When Degrapol (R) or PLGA-polymers were co-spun with increasing ratios of a water-soluble polymer that was subsequently removed; fibrous scaffolds with increased porosities were obtained. Mechanical properties correlated with fiber alignment rather than fiber diameter as aligned fiber scaffolds demonstrated strong mechanical anisotropy. For co-spun fibers the Young's modulus correlated inversely with the amount of co-spun polymer. Cell proliferation was independent of the porosity of the scaffold, but different between the two polymers. Furthermore, fibrous scaffolds with different porosities were analyzed for cell infiltration suggesting that cell infiltration was enhanced with increased porosity and increasing time. These experiments indicate that 3D-fiber fleeces can be produced with controlled properties, being prerequisites for successful scaffolds in tissue engineering applications.