Ultra performance liquid chromatography (UPLC) further improves hydrogen/deuterium exchange mass spectrometry
Ultra performance liquid chromatography (UPLC) further improves hydrogen/deuterium exchange mass spectrometry
复制标题
DOI：
10.1016/j.jasms.2005.10.009
复制
发表时间：
2006-02-01
影响因子：
3.2
通讯作者：
Hobbins, WB
中科院分区：
文献类型：
--
作者：
Wu, Y;Engen, JR;Hobbins, WB
超高效液相色谱法（UPLC）采用直径小于2 μ m的颗粒，与高效液相色谱法（HPLC）相比，可实现上级分辨率、速度和灵敏度。我们已经测试了UPLC在氢交换质谱实验中分析氘代肽的适用性。与HPLC相比，UPLC获得了上级分辨率和样品通量。对于高度氘代的模型肽，使用UPLC的氘损失大于使用常规HPLC系统观察到的氘损失，主要是由于UPLC系统的进样要求。与HPLC相比，部分氘代的细胞色素c肽在UPLC中也损失了更多的氘，尽管效果不如高度氘代的模型肽明显。UPLC的特殊色谱方面使其成为氢交换质谱实验中HPLC非常有吸引力的替代品。
Ultra performance liquid chromatography (UPLC) employs particles smaller than 2 Am in diameter to achieve superior resolution, speed, and sensitivity compared with high-performance liquid chromatography (HPLC). We have tested the suitability of UPLC for the analysis of deuterated peptides in hydrogen exchange mass spectrometry experiments. Superior resolution and sample throughput were obtained with UPLC versus HPLC. For highly deuterated model peptides, deuterium loss using UPLC was greater than the deuterium loss observed using a conventional HPLC system, primarily as a result of the injection requirements of the UPLC system. Partially deuterated cytochrome c peptides also lost more deuterium in UPLC versus HPLC, although the effect was not as pronounced as it was for the highly deuterated model peptides. The exceptional chromatographic aspects of UPLC make it a very attractive alternative to HPLC for hydrogen exchange mass spectrometry experiments.