An LC-MS/MS method for the determination of 28 polar environmental contaminants and metabolites in vegetables irrigated with treated municipal wastewater
An LC-MS/MS method for the determination of 28 polar environmental contaminants and metabolites in vegetables irrigated with treated municipal wastewater
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DOI：
10.1039/c6ay02984a
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发表时间：
2017-02-28
影响因子：
3.1
通讯作者：
Reemtsma, Thorsten
中科院分区：
文献类型：
--
作者：
Riemenschneider, Christina;Seiwert, Bettina;Reemtsma, Thorsten
建立了一种同时测定高含水量植物组织（莴苣、卷心菜和番茄）中28种废水来源污染物和可能的代谢物（如卡马西平、10，11-环氧卡马西平、苯并三唑、拉莫三嗪和双氯芬酸）的分析方法，并进行了验证。所开发的方法结合了固液萃取，而不需要一个清洁的步骤，液相色谱-串联质谱（LC-MS/MS）与电喷雾电离在积极和消极的模式。该方法与已知的QuEChERS方法进行了比较，并表现出更好的分析性能。方法在4种不同基质（甘蓝、生菜和番茄叶、番茄果实）中，对71%的目标物的检测限均达到60%以上，日内和日间精密度（RSD < 10%）均较高。与QuEChERS方法相比，所开发的方法允许测定具有不同物理化学性质（中性，酸性和碱性）的各种化合物，甚至考虑植物材料中药物的可能代谢产物。该方法已成功地应用于温室和真实的田间条件下植物体内的废水污染物及其代谢产物的分析。它可以表明，该方法适用于敏感的测定环境污染物来源于处理后的废水和一系列的代谢产物在灌溉植物及其食用部分。由于其广泛性，该提取方法还应适用于通过LC高分辨率质谱法筛选未知污染物和代谢物。
An analytical method for the simultaneous determination of 28 wastewater-derived contaminants and possible metabolites (e.g. carbamazepine, 10,11-epoxy-carbamazepine, benzotriazole, lamotrigine, and diclofenac) in common plant tissues with high water content (lettuce, cabbage, and tomato) was developed and validated. The developed method combines solid-liquid extraction, without the need for a clean-up step, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray ionization in positive and negative modes. This method was compared with the known QuEChERS method and exhibited better analytical performance. Method detection limits of 60% with high intra- and inter-day precision (RSD < 10%) could be achieved for 71% of the target analytes in four different matrices (cabbage, lettuce and tomato leaves, and tomato fruits). Compared to the QuEChERS method, the developed method allows the determination of a wide range of compounds with different physical-chemical properties (neutral, acidic, and basic) and considers even possible metabolites of pharmaceuticals in plant materials. The developed method was successfully applied to analyse wastewater-derived contaminants and their metabolites in plants which were grown under greenhouse and real field conditions. It could be shown that this method is suitable for the sensitive determination of environmental contaminants originating from treated wastewater and a range of their metabolites in irrigated plants and their edible parts. Due to its broadness the extraction method should also be suitable for screening of unknown contaminants and metabolites by LC high-resolution mass spectrometry.