Comparison of the enzyme-linked immunosorbent assay and the indirect hemagglutination and complement fixation tests for detecting antibodies to Mycoplasma hyopneumoniae.
Comparison of the enzyme-linked immunosorbent assay and the indirect hemagglutination and complement fixation tests for detecting antibodies to Mycoplasma hyopneumoniae.
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酶联免疫吸附试验与间接血凝和补体结合试验检测猪肺炎支原体抗体的比较。
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
L. Runnels
中科院分区：
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作者：
C. Armstrong;M. J. Freeman;L. Sands;M. López‐Osuna;T. Young;L. Runnels
剖宫产，初乳被剥夺的猪暴露于低传代场分离肺炎支原体通过鼻内接种肉汤培养。经鼻内接种的猪随后与它们的窝友混在一起，通过接触接触传播。在接触后大约一年的时间里，每隔两到四周收集一次猪的血清，并通过酶联免疫吸附试验（ELISA）、间接血凝和补体固定试验进行评估。鼻内暴露的猪血清转化较早，滴度较高，间接血凝和补体固定阳性的时间比接触暴露的猪长。结论鼻内暴露猪的抗体反应是人为高的，不适合用于评价猪支原体肺炎血清诊断试验的敏感性。间接血凝试验相对不敏感，技术上繁琐，作为实际的现场试验最没有希望。补体固定试验在检测早期抗体产生（特别是接触暴露的猪）方面似乎稍微敏感一些，但在检测晚期抗体方面最不敏感。ELISA通常是最敏感的方法。个体ELISA高滴度比最大补体固定和间接血凝滴度高10 ~ 32倍。三种测试之间最显著的区别是在研究后期持续的高ELISA滴度。尽管猪的肺部没有病变，培养和免疫荧光检测猪肺炎支原体呈阴性，但所有猪在接触后大约一年的尸检中均呈ELISA阳性。
Caesarean-derived, colostrum-deprived swine were exposed to a broth culture of a low passage field isolate of Mycoplasma hyopneumoniae by intranasal inoculation. The intranasal-inoculated swine subsequently were commingled with their litter-mates to effect transmission via contact-exposure. Sera were collected from the swine at two to four week intervals for approximately one year postexposure and evaluated by the enzyme-linked immunosorbent assay (ELISA), indirect hemagglutination and complement fixation tests. The intranasal-exposed swine seroconverted earlier, developed higher titers and remained indirect hemagglutination and complement fixation positive longer than the contact-exposed swine. It was concluded that the antibody response of intranasal-exposed swine was artificially high and that sera from such swine were not suitable for evaluating the sensitivity of mycoplasmal pneumonia of swine serodiagnostic tests. The indirect hemagglutination test was relatively insensitive and technically cumbersome and the least promising as a practical field test. The complement fixation test appeared to be slightly more sensitive in detecting early antibody production (especially in contact-exposed swine) but it was the least sensitive in detecting late antibodies. The ELISA was generally the most sensitive procedure. Individual high ELISA titers were from ten to 32 times greater than maximum complement fixation and indirect hemagglutination titers. The most striking difference among the three tests was the persistence of high ELISA titers late in the study. All swine were ELISA positive at necropsy approximately one year postexposure despite the fact that lungs were devoid of lesions and culturally and immunofluorescent negative for M. hyopneumoniae.