Demonstration of noradrenaline-immunoreactive nerve fibres in the liver
Demonstration of noradrenaline-immunoreactive nerve fibres in the liver
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DOI：
10.1177/030006059602400603
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发表时间：
1996-11-01
影响因子：
1.6
通讯作者：
Hayakawa, T
中科院分区：
文献类型：
--
作者：
Fukuda, Y;Imoto, M;Hayakawa, T
为了证明去甲肾上腺素在肝组织中的免疫反应神经纤维，我们使用去甲肾上腺素的抗体对大鼠、豚鼠和人的肝组织进行免疫染色。组织标本用含有焦亚硫酸钠和戊二醛的碳酸氢盐缓冲液灌流或浸泡固定，制备冰冻切片。用间接过氧化物酶标记抗体法对去甲肾上腺素进行染色。去甲肾上腺素免疫反应神经纤维定位于门脉周围血管周围和中央静脉周围。去甲肾上腺素免疫反应纤维在小叶内的分布存在种间差异。正常豚鼠和人肝小叶内可见去甲肾上腺素免疫反应纤维，而大鼠肝未见。在人类肝硬变的再生结节中，去甲肾上腺素免疫反应纤维缺失。这种通过灌流或浸泡固定直接显示去甲肾上腺素的方法适合于研究包括人类在内的各种物种的正常和受损肝脏的神经支配。
To demonstrate noradrenaline-immunoreactive nerve fibres in liver tissues, we used an antibody to noradrenaline in the immunostaining of liver tissues from rats, guinea-pigs and humans. The tissue specimens were fixed by perfusion or immersion with cacodylate buffer containing sodium metabisulphate and glutaraldehyde, and cryostat sections prepared. An indirect peroxidase-labelled antibody method was used for staining nonadrenaline. Noradrenaline-immunoreactive nerve fibres were localized around blood vessels in the portal area and around the central vein. There were differences between the species in the intralobular distribution of noradrenaline-immunoreactive fibres. Normal guinea-pig and human liver showed intralobular noradrenaline-immunoreactive fibres while rat liver did not. Noradrenaline-immunoreactive fibres were absent from regenerating nodules in a human cirrhotic liver. This method of demonstrating noradrenaline directly using perfusion- or immersion-fixation is appropriate for studying innervation in normal and damaged livers of various species including humans.