Characterization of microsatellite DNA loci in American alligators
Characterization of microsatellite DNA loci in American alligators
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作者：
Glenn, TC;Dessauer, HC;Braun, MJ
从小插入DNA文库中鉴定美洲短吻鳄的微卫星位点。微卫星的平均长度（18.4次重复）与哺乳动物相似。我们开发了聚合酶链反应（PCR）引物，并对包含至少10个不间断AC或AG重复序列的20个微卫星位点进行了测试。从路易斯安那州和佛罗里达州的短吻鳄中获得了15个容易被标记的基因型。15个位点中有11个是多态性的。多态性位点的等位基因数为4 ~ 17个（平均8.5个），群体内杂合度为0.231 ~ 0.865（平均0.466）。这些基因座的杂合性几乎是同工酶的20倍。来自路易斯安那州和佛罗里达州的人群在这些位点上差异很大（总体F-ST = 0.137, θ (P) = 0.239, R-ST = 0.387）。PCR引物还能从鳄鱼科、鳄鱼科和鳄鱼科物种中产生单扩增子，这表明它们可能对其他鳄鱼目动物的遗传研究有用。
Microsatellite loci of American alligators were identified from small insert DNA libraries. The average length of microsatellites (18.4 repeats) was similar to that observed in mammals. Polymerase chain reaction (PCR) primers were developed and tested for 20 microsatellite loci that contained at least 10 uninterrupted AC or AG repeats. Genotypes for the 15 loci that could be scored readily were obtained for alligators from Louisiana and Florida. Eleven of the 15 loci were polymorphic. For the polymorphic loci, the number of alleles per locus ranged from 4-17 (average = 8.5), and observed heterozygosity within populations ranged from 0.231-0.865 (average = 0.466). Heterozygosity of these loci is almost 20 times higher than values obtained using isozymes. Populations from Louisiana and Florida differed substantially at these loci (overall F-ST = 0.137, theta(P) = 0.239, and R-ST = 0.387). The PCR primers also produced single amplicons from species of Alligatoridae, Gavialidae, and Crocodilidae, indicating that they may be useful for genetic studies in other crocodilians.